Protocol for measuring anti-fentanyl antibodies in mouse serum by enzyme-linked immunosorbent assay.

Autor:	Koster JA; Precision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA., Prevost-Reilly A; Precision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA., Soni D; Precision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA; Global Investigative Toxicology, Preclinical Safety, Sanofi, Cambridge, MA, USA., Kelly A; Precision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA., Walker K; Precision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA., Norton EB; Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, LA 70112, USA., Levy O; Precision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA; Broad Institute of MIT & Harvard, Cambridge, MA 02142, USA., Haile CN; Department of Psychology/TIMES, University of Houston, Houston, TX 77204, USA., Dowling DJ; Precision Vaccines Program, Department of Pediatrics, Boston Children's Hospital, Boston, MA 02115, USA; Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA. Electronic address: david.dowling@childrens.harvard.edu.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2024 Dec 06; Vol. 5 (4), pp. 103402. Date of Electronic Publication: 2024 Dec 06.
DOI:	10.1016/j.xpro.2024.103402
Abstrakt:	Enzyme-linked immunosorbent assay (ELISA) offers an effective, inexpensive, and reliable approach for the analysis of humoral immune responses. Here, we describe a protocol for measuring anti-fentanyl antibodies generated by the immunization of mice with novel opioid vaccine candidates. We describe steps for coating BSA-fentanyl antigen and standard wells. We then detail procedures for preparing buffers and performing assays. This protocol is adaptable for use with either BALB/c or C57BL/6J mice strains and can measure antibody isotypes to elucidate class switching. For complete details on the use and execution of this protocol, please refer to Stone et al. 1 . Competing Interests: Declaration of interests D.S., O.L., and D.J.D. are named inventors on vaccine adjuvant patents assigned to Boston Children’s Hospital. O.L. has served as a paid consultant to GlaxoSmithKline (GSK) and HilleVax. D.J.D. is on the scientific advisory board of EdJen BioTech and serves as a consultant with Merck Research Laboratories/Merck Sharp & Dohme Corp (a subsidiary of Merck & Co., Inc.). D.J.D., O.L., and C.N.H. are cofounders and sit as scientific advisors of Ovax, Inc. E.B.N. is a scientific advisor to Ovax, Inc. These commercial or financial relationships are unrelated to the current study. C.N.H. is named as an inventor on a patent for anti-fentanyl vaccine assigned to the University of Houston. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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