The impact of endogenous N/OFQ on DPN: Insights into lower limb blood flow regulation in rats.

Autor: Qin YJ; College of Anesthesiology, Shanxi Medical University, Taiyuan 030000, China; Department of Anesthesiology, Second Hospital of Shanxi Medical University, Taiyuan 030000, China., Zhang P; College of Anesthesiology, Shanxi Medical University, Taiyuan 030000, China; Department of Anesthesiology, Second Hospital of Shanxi Medical University, Taiyuan 030000, China., Zhang P; College of Anesthesiology, Shanxi Medical University, Taiyuan 030000, China; Department of Anesthesiology, Second Hospital of Shanxi Medical University, Taiyuan 030000, China., Li J; Department of Endocrine, Central Hospital of China Railway 12th Bureau Group, 182 Yingze Road, Taiyuan 030001, Shanxi, China., Yang Q; Department of Anesthesiology, Linfen People's Hospital, Linfen 041000, China., Sun JL; College of Anesthesiology, Shanxi Medical University, Taiyuan 030000, China; Department of Anesthesiology, Second Hospital of Shanxi Medical University, Taiyuan 030000, China., Liang YZ; School of Physics, Dalian University of Technology, Dalian 116024, China., Wang LL; College of Anesthesiology, Shanxi Medical University, Taiyuan 030000, China; Department of Anesthesiology, Second Hospital of Shanxi Medical University, Taiyuan 030000, China., Zhang LZ; College of Anesthesiology, Shanxi Medical University, Taiyuan 030000, China; Department of Anesthesiology, Second Hospital of Shanxi Medical University, Taiyuan 030000, China., Han Y; College of Anesthesiology, Shanxi Medical University, Taiyuan 030000, China; Department of Anesthesiology, Second Hospital of Shanxi Medical University, Taiyuan 030000, China. Electronic address: 13753171979@163.com.
Jazyk: angličtina
Zdroj: Neuropeptides [Neuropeptides] 2024 Dec 05; Vol. 109, pp. 102492. Date of Electronic Publication: 2024 Dec 05.
DOI: 10.1016/j.npep.2024.102492
Abstrakt: Diabetic peripheral neuropathy (DPN) is a common complication of diabetes, often accompanied by impaired vascular endothelial function in the lower limbs. This dysfunction is characterized by a reduced vasodilatory response, leading to decreased blood flow in the lower limbs and ultimately contributing to the development of diabetic peripheral neuropathy. To delve deeper into this pathological process, the study employed bioinformatics to identify and analyze genes highly active in DPN. The investigation revealed that Membrane metallo-endopeptidase (MME) was effectively mitigated by its antagonist. Male Sprague-Dawley (SD) rats served as the model to systematically explore the intrinsic connection among the nociceptible/orphanin FQ-N/OFQ receptor (N/OFQ-NOP) system, femoral artery blood flow in the lower extremities, MME, and DPN. The rats were randomized into two groups: a control group and a DPN group induced by a single intraperitoneal injection of 55 mg/kg streptozotocin (STZ), with 6 rats in each group. The findings indicated that compared to the control group, the DPN group exhibited a significant reduction in femoral artery blood flow. This was accompanied by a notable increase in serum N/OFQ concentration, heightened expression of opioid-related nociceptive protein receptor 1 (OPRL1) and MME in femoral artery tissues of the lower limbs, and an elevated sciatic nerve stimulation threshold. These results suggest that the serum N/OFQ level in DPN rats is increased, which may promote the occurrence of peripheral neuropathy by up regulating MME and reducing peripheral flow distribution.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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