Bioinspired synthesis of virus-like particle-templated thin silica-layered nanocages with enhanced biocompatibility and cellular uptake as drug delivery carriers.

Autor: Kim KR; Graduate School of Biochemistry, Yeungnam University, Gyeongsan 38541, Republic of Korea., Lee AS; Graduate School of Chemical Engineering, Dongguk University, Seoul 04620, Republic of Korea., Heo HR; Department of Chemical and Biochemical Engineering, Dongguk University, Seoul 04620, Republic of Korea., Park SY; Department of Physiology, College of Medicine, Yeungnam University, Daegu 42415, Republic of Korea. Electronic address: sypark@med.yu.ac.kr., Kim CS; Graduate School of Chemical Engineering, Dongguk University, Seoul 04620, Republic of Korea; Department of Chemical and Biochemical Engineering, Dongguk University, Seoul 04620, Republic of Korea. Electronic address: biocskim@dgu.ac.kr.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2024 Nov 29; Vol. 247, pp. 114418. Date of Electronic Publication: 2024 Nov 29.
DOI: 10.1016/j.colsurfb.2024.114418
Abstrakt: The bioinspired synthesis of virus-like silica nanoparticles in biomedical applications makes it possible to utilize the cellular delivery capabilities of viruses while minimizing the cytotoxicity of inorganic silica. In this study, we developed a diatom-inspired method for synthesizing silica-layered nanocages utilizing R5 peptide-functionalized virus-like particles (VLPs). R5 peptides were genetically inserted into the F-G loop of human papillomavirus 16 L1 proteins (HPV16 L1-R5). HPV16 L1-R5 was self-assembled into VLPs under an acidic pH similar to native ones and exhibited ∼65 % drug encapsulation efficiency. The HPV16 L1-R5 VLP@silica nanocages (SiNPs) were synthesized through diatom-inspired silicification of HPV16 L1-R5 VLPs via intermolecular interaction of the R5 peptide and polyol. HPV16L1-R5 VLP@SiNPs displayed uniform, monodisperse particles with approximately 10 nm silica layer compared to HPV16 L1-R5 VLPs. HPV16 L1-R5 VLP@SiNPs showed high biocompatibility at high concentrations, unlike commercial mesoporous SiNPs. Furthermore, the virus-like HPV16 L1-R5 VLP@SiNPs resulted in approximately 2.5-fold increased cellular uptake efficiency compared to commercial mesoporous SiNPs. These results suggest that the thin silica layer on HPV16 L1-R5 VLPs retains cellular delivery capacity while reducing cytotoxicity. Our strategy presents an innovative method for synthesizing virus-like nanoparticles in biomedical applications, enhancing cellular delivery capacity and biocompatibility.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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