| Autor: |
Blaszkiewicz M; Department of Neurological Surgery, The Ohio State University, Columbus, OH, USA., Johnson CP; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, ME, USA., Willows JW; Department of Neurological Surgery, The Ohio State University, Columbus, OH, USA., Gardner ML; Department of Cancer Biology and Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.; Ohio State Biochemistry Program, Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA., Taplin DR; School of Biology and Ecology, University of Maine, Orono, ME, USA., Freitas MA; Department of Cancer Biology and Genetics, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.; Ohio State Biochemistry Program, Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA., Townsend KL; Department of Neurological Surgery, The Ohio State University, Columbus, OH, USA.; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, ME, USA.; School of Biology and Ecology, University of Maine, Orono, ME, USA.; Department of Chemical and Biomedical Engineering, University of Maine, Orono, ME, USA. |
| Abstrakt: |
White adipose tissue (WAT) is a dynamic organ capable of remodelling in response to metabolic state. For example, in response to stimuli such as cold exposure, WAT can develop inducible brown adipocytes ('browning') capable of non-shivering thermogenesis, through concurrent changes to mitochondrial content and function. This is aided by increased neurite outgrowth and angiogenesis across the tissue, providing the needed neurovascular supply for uncoupling protein 1 activation. While several RNA-sequencing studies have been performed in WAT, including newer single cell and single nuclei studies, little work has been done to investigate changes to the adipose proteome, particularly during dynamic periods of tissue remodelling such as cold stimulation. Here, we conducted a comprehensive proteomic analysis of inguinal subcutaneous (sc) WAT during the initial 'browning' period of 24 or 72hrs of cold exposure in mice. We identified four significant pathways impacted by cold stimulation that are involved in tissue remodelling, which included mitochondrial function and metabolism, cytoskeletal remodelling, the immune response, and the nervous system. Taken together, we found that early changes in the proteome of WAT with cold stimulation predicted later structural and functional changes in the tissue that are important for tissue and whole-body remodelling to meet energetic and metabolic needs. |
