A new and promising C-phycocyanin-producing cyanobacterial strain, Cyanobium sp. MMK01: practical strategy towards developing a methodology to achieve C-phycocyanin with ultra-high purity.

Autor:	Shafiei M; Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran., Shafiei M; Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran., Mohseni Sani N; Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran., Guo W; School of Medicine, Yale University, West Haven, CT, United States., Guo S; Department of Anatomy & Cell Biology, McGill University, Montreal, QC, Canada., Vali H; Department of Anatomy & Cell Biology, McGill University, Montreal, QC, Canada., Akbari Noghabi K; Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2024 Nov 21; Vol. 15, pp. 1394617. Date of Electronic Publication: 2024 Nov 21 (Print Publication: 2024).
DOI:	10.3389/fmicb.2024.1394617
Abstrakt:	Selecting a suitable cyanobacterial strain and developing easy-to-afford purification processes are two crucial aspects impacting the optimal production yield and appropriate purity of C-phycocyanin (C-PC). Cyanobium sp. MMK01, a highly efficient C-PC-producing bacterium, was identified among four cyanobacterial isolates using morphological characteristics and 16S rRNA gene sequencing. The purification process of C-PC began with ammonium sulfate precipitation, leading to a purity index (PI) of 4.04. Subsequent purification through ion exchange chromatography ultimately resulted in an ultra-highly purified form of C-PC with a significant PI of 5.82. SDS-PAGE analysis of purified C-PC showed the presence of two distinct bands, α (13 kDa) and β (15 kDa). Significantly effective at scavenging free radicals, C-PC also inhibits the viability of human lung cancer cells (Calu-6). Antibacterial, anti-inflammatory, antioxidant, and cancer-preventive compounds were detected in the MMK01 cells' methanolic extract following GC-MS analysis. The promising results indicate that Cyanobium sp. MMK01 has a great deal of potential for producing C-PC that is on par with strains found in the market, and the tried-and-true two-step purification process proved to work well to achieve an ultra-highly purified form of C-PC. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Shafiei, Shafiei, Mohseni Sani, Guo, Guo, Vali and Akbari Noghabi.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu