Methimazole-induced congenital hypothyroidism affects gonocytes differentiation and arrests meiosis: role of Sertoli cells.

Autor:	Gómez-Zúñiga A; Posgrado en Biología Experimental, Universidad Autónoma Metropolitana Unidad Iztapalapa, Mexico City, Mexico., Landero-Huerta DA; Laboratorio de Biología de la Reproducción, Instituto Nacional de Pediatría, Mexico City, Mexico., Rojas-Castañeda JC; Laboratorio de Biología de la Reproducción, Instituto Nacional de Pediatría, Mexico City, Mexico., Sánchez-Huerta K; Laboratorio de Neurociencias, Instituto Nacional de Pediatría, Mexico City, Mexico., Contreras-García IJ; Laboratorio de Biología de la Reproducción, Instituto Nacional de Pediatría, Mexico City, Mexico., Reynoso-Robles R; Laboratorio de Morfología Celular y Tisular, Instituto Nacional de Pediatría, Mexico City, Mexico., Arteaga-Silva M; Laboratorio de Neuroendocrinología Reproductiva, División de Ciencias Biológicas y de la Salud, Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana Iztapalapa, Mexico City, Mexico., Vigueras-Villaseñor RM; Laboratorio de Biología de la Reproducción, Instituto Nacional de Pediatría, Mexico City, Mexico.
Jazyk:	angličtina
Zdroj:	Frontiers in cell and developmental biology [Front Cell Dev Biol] 2024 Nov 21; Vol. 12, pp. 1493872. Date of Electronic Publication: 2024 Nov 21 (Print Publication: 2024).
DOI:	10.3389/fcell.2024.1493872
Abstrakt:	Background: Congenital hypothyroidism (CH) is a pathology that affects various organs, including the testicles. The mechanisms by which this condition alters fertility is unknown. This study aimed at determining if experimental CH affects gonocyte differentiation and arrests meiosis; and the possible role of the Sertoli cell (SC) in this condition. Material and Methods: Two groups of rats consisting of Control group and Methimazole (MMI) induced CH rats were formed. The induction of CH was achieved by the administration of MMI starting on day 16 postcoitum and continued until euthanized. Euthanasia was performed at 5, 8, 10, 16 and 64 days of age. Following this, the testicular tissue of each animal was extracted and processed for histopathological and ultrastructural analysis. In addition, the tissue was used for the determination of proteins and their transcriptions, events which are characteristics of gonocyte differentiation. The SC functionality proteins was determined immunohistochemically, while sperm parameters of the cauda epididymis were verified. Results: CH caused a delay in the gonocyte differentiation, and arrested meiosis and spermiogenesis. These events had long-term repercussions on the quality of the seminiferous epithelium. The results show that CH induces alterations in the functional state of SCs that may have led to the deficiency in the synthesis and/or in the release of molecules necessary for gonocyte differentiation; as well as disorders in the process of meiosis that resulted in sperm absence. Conclusion: These results suggest that CH affects gonocyte differentiation and arrests meiosis, possibly through altering the functional status of SCs. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Gómez-Zúñiga, Landero-Huerta, Rojas-Castañeda, Sánchez-Huerta, Contreras-García, Reynoso-Robles, Arteaga-Silva and Vigueras-Villaseñor.)
Databáze:	MEDLINE
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