Characterization of class-switched B cells in chickens.

Autor:	von La Roche D; Department of Veterinary Sciences, AG Immunology, Ludwig-Maximilians-Universität München, Planegg, Germany., Schumacher M; Department of Veterinary Sciences, AG Immunology, Ludwig-Maximilians-Universität München, Planegg, Germany., Kohn M; Department of Veterinary Sciences, AG Immunology, Ludwig-Maximilians-Universität München, Planegg, Germany., Trapp J; Clinic for Poultry, University of Veterinary Medicine Hannover, Hannover, Germany., Schusser B; Department of Veterinary Sciences, AG Immunology, Ludwig-Maximilians-Universität München, Planegg, Germany.; Reproductive Biotechnology, TUM School of Life Sciences, Technische Universität München, Freising, Germany.; Center of Infection Prevention (ZIP), Technische Universität München, Freising, Germany., Rautenschlein S; Clinic for Poultry, University of Veterinary Medicine Hannover, Hannover, Germany., Härtle S; Department of Veterinary Sciences, AG Immunology, Ludwig-Maximilians-Universität München, Planegg, Germany.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2024 Nov 21; Vol. 15, pp. 1484288. Date of Electronic Publication: 2024 Nov 21 (Print Publication: 2024).
DOI:	10.3389/fimmu.2024.1484288
Abstrakt:	While B cell development in the birds' primary B cell organ, the bursa Fabricius, is relatively well understood, very little is known about post bursal B cell differentiation into plasma and memory cells though these cells are essential for a protecting antibody response and so far, no specific markers for these cells were available. Since immunoglobulin class switch is one part of the B cell differentiation process, our objective was to conduct a first detailed investigation of class-switched chicken B cells. As only very few IgY and IgA expressing cells were detected in lymphoid organs of young chickens, we used CD40L and IL-10 to establish a prolonged in vitro culture system, which induces B cell proliferation, class switch to IgY and IgA and enhanced antibody secretion. This enabled a phenotypic analysis of differentiating B cells. Importantly, these cells lost surface expression of the B cell markers chB6 and BAFF-R. B cell receptor surface expression remained unchanged, showing that while differentiating toward plasma cells, B cells can be addressed by L chain staining. Newly generated potential plasma cell markers CD138 and TACI showed only a transient expression on cultured cells and rather act as markers for B cell activation than plasma/memory cells in general. CD57 upregulation was connected to activation and blast formation but not to class switch. We also examined potential changes in class-switched cells in different age groups and post vaccination. Surprisingly, bursa involution, laying and age had no distinct effects on the presence of class-switched cells, but we detected significantly more class-switched B cells post vaccination. Hence, we are now able to generate class-switched plasmablasts in vitro for a more detailed characterization and can address them under different conditions in chickens for further analysis of their B cell response. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 von La Roche, Schumacher, Kohn, Trapp, Schusser, Rautenschlein and Härtle.)
Databáze:	MEDLINE
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