High Occurrence of ST131 Among Extraintestinal Pathogenic Escherichia coli in Humans and Dogs in Sri Lanka.

Autor: Dewasmika WAPM; Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya Sri Lanka, Peradeniya, Sri Lanka., Dissanayake DRA; Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya Sri Lanka, Peradeniya, Sri Lanka., Neelawala NGDAK; Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya Sri Lanka, Peradeniya, Sri Lanka., Dissanayake BGN; Department of Microbiology, Faculty of Medicine University of Peradeniya, Peradeniya, Sri Lanka., Prasada DVP; Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka., Francis VR; Department of Pathophysiology, Faculty of Healthcare Sciences, Eastern University, Batticaloa, Sri Lanka., Octavia S; School of Biotechnology and Biomolecular Sciences, University of new South Wales, Sydney, New South Wales, Australia., Lan R; School of Biotechnology and Biomolecular Sciences, University of new South Wales, Sydney, New South Wales, Australia.
Jazyk: angličtina
Zdroj: Zoonoses and public health [Zoonoses Public Health] 2024 Dec 05. Date of Electronic Publication: 2024 Dec 05.
DOI: 10.1111/zph.13199
Abstrakt: Aims: Extraintestinal pathogenic Escherichia coli (ExPEC) is an important human and animal pathogen. In particular, the ST131 of ExPEC is a widely distributed clone, resistant to extended-spectrum cephalosporins and fluoroquinolones. We investigated the occurrence of ST131 among ExPEC from humans and dogs in Sri Lanka and determined its antimicrobial resistance.
Methods: A total of 215 ExPEC isolates were collected from humans (n = 179) and dogs (n = 36) from two different cities in Sri Lanka. Antimicrobial resistance was tested by the disk diffusion method, and the presence of resistance-encoding genes (bla TEM , bla SHV and bla CTX-M ) and ST131 clades/subclades was tested by PCR.
Results: The majority of the isolates were (61.8%) ST131, with 55.8% in ST131-clade C. Of the clade C isolates, 44.3%, 27.8%, 15.7% and 6.7% were in clades C2, C other, C1-non-M27 and C1-M27, respectively. Approximately 11% of the ST131 isolates were carbapenem resistant. ExPEC from dogs showed comparable resistance rates to human isolates except for resistance to amoxicillin-clavulanate and amikacin.
Conclusion: We observed a high occurrence of ST131 and its clade C, with more than 11% exhibiting resistance to carbapenems in Sri Lanka. Furthermore, ST131-C1-M27, with high resistance to both quinolones and extended-spectrum cephalosporins, was also present. Our results emphasise the importance of the One Health approach in the monitoring of antimicrobial-resistant E. coli in different regions of Sri Lanka to gain a better understanding of their prevalence over time, contributing to effective antimicrobial stewardship.
(© 2024 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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