Molecular sociology of virus-induced cellular condensates supporting reovirus assembly and replication.

Autor: Liu X; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, USA.; California NanoSystems Institute, University of California, Los Angeles, CA, USA., Xia X; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, USA.; California NanoSystems Institute, University of California, Los Angeles, CA, USA., Martynowycz MW; Howard Hughes Medical Institute, University of California, Los Angeles, CA, USA.; Department of Biological Chemistry, University of California, Los Angeles, CA, USA.; Hauptman-Woodward Medical Research Institute, Buffalo, NY, USA., Gonen T; Howard Hughes Medical Institute, University of California, Los Angeles, CA, USA.; Department of Biological Chemistry, University of California, Los Angeles, CA, USA., Zhou ZH; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, USA. Hong.Zhou@UCLA.edu.; California NanoSystems Institute, University of California, Los Angeles, CA, USA. Hong.Zhou@UCLA.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Dec 06; Vol. 15 (1), pp. 10638. Date of Electronic Publication: 2024 Dec 06.
DOI: 10.1038/s41467-024-54968-7
Abstrakt: Virus-induced cellular condensates, or viral factories, are poorly understood high-density phases where replication of many viruses occurs. Here, by cryogenic electron tomography (cryoET) of focused ion beam (FIB) milling-produced lamellae of mammalian reovirus (MRV)-infected cells, we visualized the molecular organization and interplay (i.e., "molecular sociology") of host and virus in 3D at two time points post-infection, enabling a detailed description of these condensates and a mechanistic understanding of MRV replication within them. Expanding over time, the condensate fashions host ribosomes at its periphery, and host microtubules, lipid membranes, and viral molecules in its interior, forming a 3D architecture that supports the dynamic processes of viral genome replication and capsid assembly. A total of six MRV assembly intermediates are identified inside the condensate: star core, empty and genome-containing cores, empty and full virions, and outer shell particle. Except for star core, these intermediates are visualized at atomic resolution by cryogenic electron microscopy (cryoEM) of cellular extracts. The temporal sequence and spatial rearrangement among these viral intermediates choreograph the viral life cycle within the condensates. Together, the molecular sociology of MRV-induced cellular condensate highlights the functional advantage of transient enrichment of molecules at the right location and time for viral replication.
Competing Interests: Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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