Immunohistochemical assessment of ERM proteins (ezrin, radixin, moesin) in the ovaries of different species.

Autor: Leitner N; Department of Biomedical Sciences and Pathobiology, Center of Pathobiology, University of Veterinary Medicine, Vienna, Austria. Electronic address: natascha.leitner@vetmeduni.ac.at., Simsek I; Department of Biomedical Sciences and Pathobiology, Center of Pathobiology, University of Veterinary Medicine, Vienna, Austria. Electronic address: ismi.simsek@gmx.at., Hlavaty J; Department of Biomedical Sciences and Pathobiology, Center of Pathobiology, University of Veterinary Medicine, Vienna, Austria. Electronic address: Juraj.hlavaty@vetmeduni.ac.at., Schäfer-Somi S; Department of Small Animals and Horses, Clinical Center for Reproduction University of Veterinary Medicine, Vienna, Austria. Electronic address: sabine.schaefer@vetmeduni.ac.at., Walter I; Department of Biomedical Sciences and Pathobiology, Center of Pathobiology, University of Veterinary Medicine, Vienna, Austria; VetCore Facility for Research, University of Veterinary Medicine, Vienna, Austria. Electronic address: Ingrid.walter@vetmeduni.ac.at.
Jazyk: angličtina
Zdroj: Tissue & cell [Tissue Cell] 2024 Nov 30; Vol. 93, pp. 102644. Date of Electronic Publication: 2024 Nov 30.
DOI: 10.1016/j.tice.2024.102644
Abstrakt: The ezrin/radixin/moesin proteins play a central role in cross-linking plasma membrane proteins with the actin cytoskeleton. Despite intensive ERM protein research in many tissues and pathologies, little is known about these proteins in healthy tissues of reproductive organs. Therefore, we examined ezrin, phosphorylated ezrin/radixin/moesin (pan-pERM), radixin, and moesin distribution at the cellular level by means of immunohistochemistry in ovaries of the following animal species: mouse, dog, cat, sheep, pig, horse, and cynomolgus monkey. Ezrin was expressed in oocytes, ovarian surface, granulosa cells and corpus luteum. A characteristic, predominantly membranous pan-pERM staining pattern was observed in ovarian surface epithelium, oocyte, granulosa cells and corpus luteum. Moesin immunoreactivity was present in all ovarian structures with a prominent signal in endothelial cells of blood vessels. Oocytes, granulosa cells and corpus luteum revealed mainly nuclear radixin staining. Staining pattern and subcellular localization (membranous, cytoplasmic, nuclear) varied between different animal species and between particular ERM proteins as well. This data may help gain new insights into the physiological function of ERM proteins in biological events in the female reproductive system.
Competing Interests: Declaration of Competing Interest The authors have nothing to declare.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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