Active-reset protein sensors enable continuous in vivo monitoring of inflammation.

Autor:	Zargartalebi H; Department of Biomedical Engineering, McCormick School of Engineering, Northwestern University, Evanston, IL, USA.; Department of Chemistry, Weinberg College of Arts and Sciences, Northwestern University, Evanston, IL, USA.; Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada., Mirzaie S; Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada., GhavamiNejad A; Department of Biomedical Engineering, McCormick School of Engineering, Northwestern University, Evanston, IL, USA., Ahmed SU; Department of Biomedical Engineering, McCormick School of Engineering, Northwestern University, Evanston, IL, USA.; Robert H. Laurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA., Esmaeili F; Department of Chemistry, Weinberg College of Arts and Sciences, Northwestern University, Evanston, IL, USA.; Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON, Canada., Geraili A; Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada., Flynn CD; Department of Chemistry, Weinberg College of Arts and Sciences, Northwestern University, Evanston, IL, USA., Chang D; Department of Biomedical Engineering, McCormick School of Engineering, Northwestern University, Evanston, IL, USA., Das J; Department of Chemistry, Weinberg College of Arts and Sciences, Northwestern University, Evanston, IL, USA., Abdrabou A; Robert H. Laurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA.; Chan Zuckerberg Biohub Chicago, Chicago, IL, USA., Sargent EH; Department of Chemistry, Weinberg College of Arts and Sciences, Northwestern University, Evanston, IL, USA.; Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON, Canada.; Department of Electrical and Computer Engineering, Northwestern University, Evanston, IL, USA., Kelley SO; Department of Biomedical Engineering, McCormick School of Engineering, Northwestern University, Evanston, IL, USA.; Department of Chemistry, Weinberg College of Arts and Sciences, Northwestern University, Evanston, IL, USA.; Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada.; Robert H. Laurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA.; Chan Zuckerberg Biohub Chicago, Chicago, IL, USA.
Jazyk:	angličtina
Zdroj:	Science (New York, N.Y.) [Science] 2024 Dec 06; Vol. 386 (6726), pp. 1146-1153. Date of Electronic Publication: 2024 Dec 05.
DOI:	10.1126/science.adn2600
Abstrakt:	Continuous measurement of proteins in vivo is important for real-time disease management and prevention. Implantable sensors for monitoring small molecules such as glucose have been available for more than a decade. However, analysis of proteins remains an unmet need because the lower physiological levels require that sensors have high affinities, which are linked to long complexation half-lives ( t 1/2 ~20 hours) and slow equilibration when concentrations decrease. We report active-reset sensors by use of high-frequency oscillations to accelerate dissociation, which enables regeneration of the unbound form of the sensor within 1 minute. When implemented within implanted devices, these sensors allow for real-time, in vivo monitoring of proteins within interstitial fluid. Active-reset protein sensors track biomarker levels on a physiological timescale for inflammation monitoring in living animals.
Databáze:	MEDLINE
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