Simultaneous visualization of membrane fluidity and morphology defines adhesion signatures of cancer cells.

Autor:	Matsuzaki T; Department of Applied Physics, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan.; Division of Precision Engineering and Applied Physics, Center for Future Innovation, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan., Fujii M; Department of Chemistry, Saitama University, Sakura-Ku, Saitama 338-8570, Japan., Noro H; Department of Chemistry, Saitama University, Sakura-Ku, Saitama 338-8570, Japan., Togo S; Department of Chemistry, Saitama University, Sakura-Ku, Saitama 338-8570, Japan., Watanabe M; Department of Chemistry, Saitama University, Sakura-Ku, Saitama 338-8570, Japan., Suganuma M; Division of Strategic Research and Development, Graduate School of Science and Engineering, Saitama University, Sakura-Ku, Saitama 338-8570, Japan., Sharma S; Directorate of Engineering, US National Science Foundation, Alexandria, VA 22314., Kobayashi N; Division of Strategic Research and Development, Graduate School of Science and Engineering, Saitama University, Sakura-Ku, Saitama 338-8570, Japan., Kawamura R; Department of Chemistry, Saitama University, Sakura-Ku, Saitama 338-8570, Japan., Nakabayashi S; Department of Chemistry, Saitama University, Sakura-Ku, Saitama 338-8570, Japan.; Division of Strategic Research and Development, Graduate School of Science and Engineering, Saitama University, Sakura-Ku, Saitama 338-8570, Japan., Yoshikawa HY; Department of Applied Physics, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Dec 10; Vol. 121 (50), pp. e2412914121. Date of Electronic Publication: 2024 Dec 05.
DOI:	10.1073/pnas.2412914121
Abstrakt:	We developed an advanced optical microscope for the simultaneous visualization of membrane fluidity and morphology to define cell adhesion signatures. This microscope combines ratiometric spectral imaging of membrane fluidity and interferometric imaging of membrane morphology. As a preliminary demonstration, we simultaneously visualized the interface between a giant unilamellar vesicle (GUV) and a glass substrate at different temperatures. We identified more fluid regions of the membrane and membrane adhesion sites (conversely, low-fluidic, ordered membrane domains correlate with nonadhered regions). This microscopic system was applied to human breast cancer cell lines with different malignancies; then, we identified adhesion signature of cancer cells: 1) low-fluidic, ordered membrane domains at the cell periphery and 2) large fluidic deviation at the nonadhered region. Inhibition of the cholesterol synthesis pathway suppresses the ordered membrane domains at the cancer cell periphery; thus, high level of cholesterol supports the appearance. Furthermore, an inhibitor of the unsaturated lipid synthesis pathway suppressed the large fluidic deviation at the nonadhered region; variation of unsaturated lipids contributes to heterogeneity of the cancer membrane. Therefore, our advanced optical microscopy enables us to couple membrane physical properties with cell adhesion, leading to definition of adhesion signatures of broad cell types, not just for cancer cells, that regulate life phenomena. Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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