In vitro tear replenishment system: assessing drug delivery from contact lens biomaterials through corneal epithelial monolayer and multilayer under replenishment conditions.

Autor: Mohammadi S; Systems Design Engineering, University of Waterloo, 200 University Ave W, Waterloo, ON, N2L 3G1, Canada., Eslami S; Systems Design Engineering, University of Waterloo, 200 University Ave W, Waterloo, ON, N2L 3G1, Canada., Jones L; Centre for Ocular Research and Education (CORE), School of Optometry and Vision Science, University of Waterloo, 200 University Ave W, Waterloo, ON, N2L 3G1, Canada., Gorbet M; Systems Design Engineering, University of Waterloo, 200 University Ave W, Waterloo, ON, N2L 3G1, Canada. mgorbet@uwaterloo.ca.; Centre for Ocular Research and Education (CORE), School of Optometry and Vision Science, University of Waterloo, 200 University Ave W, Waterloo, ON, N2L 3G1, Canada. mgorbet@uwaterloo.ca.
Jazyk: angličtina
Zdroj: Drug delivery and translational research [Drug Deliv Transl Res] 2024 Dec 05. Date of Electronic Publication: 2024 Dec 05.
DOI: 10.1007/s13346-024-01746-z
Abstrakt: There is a need to develop improved in vitro ocular models for biocompatibility and drug delivery studies to assess the potential of in vivo performance of contact lenses. By using an in vitro corneal epithelial cell model combined with a tear replenishment method, this study aimed to investigate the delivery of the glaucoma drug latanoprost from contact lenses and compare the dynamic release results to no-replenishment (immersion) conditions. Corneal epithelial cells were grown as a monolayer or multilayer on curved cellulose cell culture inserts. Three contact lens materials (balafilcon A; senofilcon A; etafilcon A), soaked for 24 h in latanoprost, were placed on the curved cornea models (CCM) and drug concentration was determined on the basal (diffusion/transport) and apical (supernatant) sides after 1, 4, 8 and 12 h. The in vitro tear replenishment was achieved via intermittent flow of a tear solution over the CCM at a rate of 1 mL/hour. A zero-order kinetic was observed for basal drug concentration over the 12 h period. Similar basal and apical drug concentrations were observed with monolayer and multilayer CCM, except for the etafilcon A material. The apical release of latanoprost was significantly lower under replenishment compared to no-replenishment conditions. These results demonstrate the role that a dynamic release model will have in predicting the amount of drug that can be released from a contact lens into the tear film and the critical role of a cell monolayer in in vitro drug delivery studies.
Competing Interests: Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: All authors agree to publish this article in Drug Delivery and Translational Research. Competing interests: The authors have no relevant financial or non-financial interests to disclose.
(© 2024. The Author(s).)
Databáze: MEDLINE
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