Surface plasmon polariton-enhanced upconversion luminescence for biosensing applications.

Autor:	Le D; Sensing Solutions, VTT Technical Research Centre of Finland, 90570 Oulu, Finland., Kreivi M; Sensing Solutions, VTT Technical Research Centre of Finland, 90570 Oulu, Finland., Aikio S; Sensing Solutions, VTT Technical Research Centre of Finland, 90570 Oulu, Finland., Heinilehto N; Sensing Solutions, VTT Technical Research Centre of Finland, 90570 Oulu, Finland., Sipola T; Sensing Solutions, VTT Technical Research Centre of Finland, 90570 Oulu, Finland., Petäjä J; Sensing Solutions, VTT Technical Research Centre of Finland, 90570 Oulu, Finland., Guo TL; Department of Physics and Mathematics, University of Eastern Finland, P.O. Box 111, FI-80101 Joensuu, Finland., Roussey M; Department of Physics and Mathematics, University of Eastern Finland, P.O. Box 111, FI-80101 Joensuu, Finland., Hiltunen J; Sensing Solutions, VTT Technical Research Centre of Finland, 90570 Oulu, Finland.
Jazyk:	angličtina
Zdroj:	Nanophotonics (Berlin, Germany) [Nanophotonics] 2024 Aug 27; Vol. 13 (21), pp. 3995-4006. Date of Electronic Publication: 2024 Aug 27 (Print Publication: 2024).
DOI:	10.1515/nanoph-2024-0247
Abstrakt:	Upconversion luminescence (UCL) has great potential for highly sensitive biosensing due to its unique wavelength shift properties. The main limitation of UCL is its low quantum efficiency, which is typically compensated using low-noise detectors and high-intensity excitation. In this work, we demonstrate surface plasmon polariton (SPP)-enhanced UCL for biosensing applications. SPPs are excited by using a gold grating. The gold grating is optimized to match the SPP resonance with the absorption wavelength of upconverting nanoparticles (UCNPs). Functionalized UCNPs conjugated with antibodies are immobilized on the surface of the fabricated gold grating. We achieve an UCL enhancement up to 65 times at low excitation power density. This enhancement results from the increase in the absorption cross section of UCNPs caused by the SPP coupling on the grating surface. Computationally, we investigated a slight quenching effect in the emission process with UCNPs near gold surfaces. The experimental observations were in good agreement with the simulation results. The work enables UCL-based assays with reduced excitation intensity that are needed, for example, in scanning-free imaging. Competing Interests: Conflict of interest: Authors state no conflict of interest. (© 2024 the author(s), published by De Gruyter, Berlin/Boston.)
Databáze:	MEDLINE
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