Unlocking Probiotic Potential: Genomic Insights into Weissella paramesenteroides UFTM 2.6.1.

Autor: Rocha BMO; Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Brazil., Sabino YNV; Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Brazil., de Almeida TC; Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Brazil., Palacio FB; Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Brazil., Rotta IS; Universidade Federal Do Triângulo Mineiro (UFTM), Uberaba, Brazil., Dias VC; Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Brazil., da Silva VL; Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Brazil., Diniz CG; Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Brazil., Azevedo VAC; Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil., Brenig B; Georg August University, Göttingen, Germany., Soares SC; Universidade Federal Do Triângulo Mineiro (UFTM), Uberaba, Brazil., Paiva AD; Universidade Federal Do Triângulo Mineiro (UFTM), Uberaba, Brazil., Medeiros JD; Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Brazil., Machado ABF; Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, Brazil. alessandra.machado@ufjf.br.
Jazyk: angličtina
Zdroj: Probiotics and antimicrobial proteins [Probiotics Antimicrob Proteins] 2024 Dec 05. Date of Electronic Publication: 2024 Dec 05.
DOI: 10.1007/s12602-024-10409-x
Abstrakt: Weissella, a genus of lactic acid bacteria, has diverse beneficial attributes including probiotic activity and biotechnological applications. Therefore, the investigation of the Weissella genus has garnered growing interest. In this study, we sequenced the complete genome of Weissella paramesenteroides UFTM 2.6.1 isolated from unpasteurized cow's milk from the Triângulo Mineiro region and performed probiogenomic analyses. Taxonomic characterization confirmed the identity of W. paramesenteroides. The genome comprises 1926 protein-coding genes, mainly related to cell metabolism, information storage and processing, and cellular processes and signaling. Ninety-nine unique genes associated with probiotic functions were identified in the genome of W. paramesenteroides UFTM 2.6.1, including genes involved in stress response, bacterial persistence in the gastrointestinal tract, and biosynthesis of vitamins. In silico analysis of bacteriocin-related genes identified Pediocin, and subsequent in vitro testing confirmed that W. paramesenteroides UFTM 2.6.1 exhibits antimicrobial activity against Listeria spp. Genomic characterization revealed the presence of the replicon pLCK4 and four prophage regions, one of which was intact. Moreover, no CRISPR-Cas array or associated Cas proteins were found, along with an absence of resistance and virulence genes, suggesting a safety aspect of the evaluated strain. Pan-genome analysis unveiled 204 exclusive genes in the genome of W. paramesenteroides UFTM 2.6.1, which includes metabolism and stress-associated genes. In general, the results indicate probiotic potential of W. paramesenteroides UFTM 2.6.1. Further studies are required to ensure the safety and beneficial effects of this bacterium in vivo, aiming for future applications in the food industry and animal and human medicine.
Competing Interests: Declarations. Competing Interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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