Exon disruptive variants in Populus trichocarpa associated with wood properties exhibit distinct gene expression patterns.

Autor: Piot A; Department of Wood and Forest Sciences, Université Laval, Quebec City, Quebec, Canada.; Institute for System and Integrated Biology (IBIS), Université Laval, Quebec City, Quebec, Canada.; Centre for Forest Research, Université Laval, Quebec City, Quebec, Canada., El-Kassaby YA; Department of Forest and Conservation Sciences, Faculty of Forestry, The University of British Columbia, Vancouver, British Columbia, Canada., Porth I; Department of Wood and Forest Sciences, Université Laval, Quebec City, Quebec, Canada.; Institute for System and Integrated Biology (IBIS), Université Laval, Quebec City, Quebec, Canada.; Centre for Forest Research, Université Laval, Quebec City, Quebec, Canada.
Jazyk: angličtina
Zdroj: The plant genome [Plant Genome] 2024 Dec 04, pp. e20541. Date of Electronic Publication: 2024 Dec 04.
DOI: 10.1002/tpg2.20541
Abstrakt: Forest trees may harbor naturally occurring exon disruptive variants (DVs) in their gene sequences, which potentially impact important ecological and economic phenotypic traits. However, the abundance and molecular regulation of these variants remain largely unexplored. Here, 24,420 DVs were identified by screening 1014 Populus trichocarpa full genomes. The identified DVs were predominantly heterozygous with allelic frequencies below 5% (only 26% of DVs had frequencies greater than 5%). Using common garden-grown trees, DVs were assessed for gene expression variation in the developing xylem, revealing that their gene expression can be significantly altered, particularly for homozygous DVs (in the range of 27%-38% of cases depending on the studied common garden). DVs were further investigated for their correlations with 13 wood quality traits, revealing that, among the 148 discovered DV associations, 15 correlated with more than one wood property and six genes had more than one DV in their coding sequences associated with wood traits. Approximately one-third of DVs correlated with wood property variation also showed significant gene expression variation, confirming their non-spurious impact. These findings offer potential avenues for targeted introduction of homozygous mutations using tree biotechnology, and while the exact mechanisms by which DVs may directly influence wood formation remain to be unraveled, this study lays the groundwork for further investigation.
(© 2024 The Author(s). The Plant Genome published by Wiley Periodicals LLC on behalf of Crop Science Society of America.)
Databáze: MEDLINE
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