| Autor: |
Zhao JY; Institute of Infectious Disease Control and Prevention,Henan Provincial Center for Disease Control and Prevention, Zhengzhou450016, China., Song WR; Institute of Infectious Disease Control and Prevention,Henan Provincial Center for Disease Control and Prevention, Zhengzhou450016, China., Zhang BF; Institute of Infectious Disease Control and Prevention,Henan Provincial Center for Disease Control and Prevention, Zhengzhou450016, China., Hu YX; Institute of Infectious Disease Control and Prevention,Henan Provincial Center for Disease Control and Prevention, Zhengzhou450016, China., Li YF; Institute of Infectious Disease Control and Prevention,Henan Provincial Center for Disease Control and Prevention, Zhengzhou450016, China., Pan JJ; Institute of Infectious Disease Control and Prevention,Henan Provincial Center for Disease Control and Prevention, Zhengzhou450016, China., Wang HF; Institute of Infectious Disease Control and Prevention,Henan Provincial Center for Disease Control and Prevention, Zhengzhou450016, China., Guo WS; Institute of Infectious Disease Control and Prevention,Henan Provincial Center for Disease Control and Prevention, Zhengzhou450016, China., Huang XY; Institute of Infectious Disease Control and Prevention,Henan Provincial Center for Disease Control and Prevention, Zhengzhou450016, China., Ye Y; Institute of Infectious Disease Control and Prevention,Henan Provincial Center for Disease Control and Prevention, Zhengzhou450016, China. |
| Jazyk: |
čínština |
| Zdroj: |
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi [Zhonghua Liu Xing Bing Xue Za Zhi] 2024 Nov 10; Vol. 45 (11), pp. 1559-1565. |
| DOI: |
10.3760/cma.j.cn112338-20240507-00244 |
| Abstrakt: |
Objective: To analyze the genus, drug resistance/virulence and phylogenetic characteristics of Brucella strains isolated from brucellosis surveillance sentinels in Henan Province from 2013 to 2022, and provide baseline data for the surveillance, early warning and outbreak tracing of brucellosis. Methods: Blood samples were collected from patients with Brucella infection for strain isolation, culture and species identification, drug susceptibility test, whole genome sequencing, splicing and assembly, functional/virulence/resistance gene prediction analysis and phylogenetic tree drawing based on single nucleotide polymorphism (SNP). Results: In 36 brucellosis patients, the majority were men (86.11%, 31/36), young adults aged 18-50 (88.89%, 32/36) and farmers/herdsmen (72.22%, 26/36). A total of 36 strains of Brucella melitensis were isolated, and average 1 305 functional proteins of 21 categories were predicted by strain genome; all the strains carried four main virulence factors (pmm, VirB group, BtpA/BtpB, BvrS/BvrR). The drug sensitivity rate was 100.00% to six types of antibiotics including levofloxacin, rifampicin, doxycycline, streptomycin, tetracycline and gentamicin, they showed different resistances to three antibiotics including compound trimethoprim-sulfamethoxazole, ciprofloxacin and ampicillin. The strains carried four types of resistance genes and two clusters of resistance genes, with four combinations of genotypes, the resistance mechanisms included antibiotic degradation/modification enzymes, resistant nodular cell differentiation (RND) efflux pumps, 16S/23S ribosomal rRNA binding site mutations, etc . The number of SNP differed in the genomes of 36 Brucella melitensis strains ranged from 0 to 454 and phylogenetic tree was divided into three major branches, with relative branch distances between 0.000 0 and 0.498 6 for each strain. Conclusions: Human Brucella melitensis strains isolated from surveillance sentinels in Henan from 2013 to 2022 carried multiple virulence and antibiotic resistance genes and had different drug resistance phenotypes. Single nucleotide polymorphism analysis and phylogenetic tree analysis showed significant differences in phylogenetic relationships among different strains. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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