The cyclization of human salivary Histatin 1 via click chemistry for skin wound healing.

Autor: Lei X; Dermatology Hospital, Southern Medical University, Guangzhou, China., Yang Y; Department of Burn and Plastic Surgery, General Hospital of Southern Theater Command, Guangzhou, China; Department of Plastic Surgery, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Zheng J; Dermatology Hospital, Southern Medical University, Guangzhou, China., Liang L; Dermatology Hospital, Southern Medical University, Guangzhou, China., Cheng L; Department of Burn and Plastic Surgery, General Hospital of Southern Theater Command, Guangzhou, China., Dong Y; Department of Burn and Plastic Surgery, General Hospital of Southern Theater Command, Guangzhou, China., Qiu B; Dermatology Hospital, Southern Medical University, Guangzhou, China., Bikker FJ; Department of Oral Biochemistry, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam (UvA) and Vrije Universiteit Amsterdam (VU), Amsterdam, The Netherlands., Forouzanfar T; Department of Oral and Maxillofacial Surgery/Pathology, Amsterdam UMC and Academic Center for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam, Amsterdam Movement Science, Amsterdam, The Netherlands., Cheng B; Dermatology Hospital, Southern Medical University, Guangzhou, China. Electronic address: chengbiaocheng@163.com., Wu G; Savaid Stomatology School, Hangzhou Medical college, Hangzhou, Zhejiang Province, PR China. Electronic address: g.wu@acta.nl., Yang B; Dermatology Hospital, Southern Medical University, Guangzhou, China. Electronic address: yangbin1@smu.edu.cn.
Jazyk: angličtina
Zdroj: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences [Eur J Pharm Sci] 2025 Jan 01; Vol. 204, pp. 106978. Date of Electronic Publication: 2024 Dec 02.
DOI: 10.1016/j.ejps.2024.106978
Abstrakt: Acute skin injuries can result in the breakdown of the skin barrier, heightening the risk of infections and complications. Histatin 1 (Hst1) promotes the adhesion, spreading, and migration of various skin-related cells, thus encouraging wound healing. However, Hst1 is extensively degraded upon exposure to wound exudates. Cyclized hst1 (Cyclic-hst1) has a much higher resistance to protease degradation than Hst1, thus increasing its stability and half-life. Herein, we synthesized Cyclic-hst1 via a click reaction and explored its efficacy in wound healing via cellular and animal experiments. Cyclic-hst1, at a 100-fold lower concentration than Hst1, effectively promoted acute skin wound healing. In addition, Cyclic-hst1 had a superior effect to Hst1 in terms of its anti-inflammatory, re-epithelialization, collagen deposition, and angiogenic effects, thus significantly promoting skin wound healing. Consequently, Cyclic-hst1 could represent a favorable treatment to manage acute skin wound healing, providing a promising experimental basis for clinical transformation and application.
Competing Interests: Declaration of competing interest All authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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