Women with pelvic organ prolapse and fibulin-5 rs12589592 polymorphism.

Autor:	Maeda FGR; Faculty of Medicine of ABC, Molecular Biology Laboratory - Santo André (SP), Brazil., Palos CC; Faculty of Medicine of ABC, Department of Gynecology and Obstetrics - Santo André (SP), Brazil., Fernandes CE; Faculty of Medicine of ABC, Department of Gynecology and Obstetrics - Santo André (SP), Brazil., Souto RPD; Faculty of Medicine of ABC, Department of Biochemistry - Santo André (SP), Brazil., Oliveira E; Faculty of Medicine of ABC, Department of Gynecology and Obstetrics - Santo André (SP), Brazil.
Jazyk:	angličtina
Zdroj:	Revista da Associacao Medica Brasileira (1992) [Rev Assoc Med Bras (1992)] 2024 Dec 02; Vol. 70 (11), pp. e20240687. Date of Electronic Publication: 2024 Dec 02 (Print Publication: 2024).
DOI:	10.1590/1806-9282.20240687
Abstrakt:	Objective: This study aims to access the frequency of single-nucleotide polymorphism rs12589592 (G>A) of the fibulin-5 gene in a Brazilian population with pelvic organ prolapse. Methods: This was a case-control study, with menopausal women divided into two groups and classified using the pelvic organ prolapse quantification system: pelvic organ prolapse group: pelvic organ prolapse quantification system stages III and IV and Control group: pelvic organ prolapse quantification system stages I and 0. We collected epidemiologic and baseline health information and performed genotyping of rs12589592 from the fibulin-5 gene using a restriction fragment length polymorphism (polymerase chain reaction-restriction fragment length polymorphism) strategy, based on the distinction of sequences from alleles G and A by the restriction enzyme DdeI. For the pelvic organ prolapse group and control, 111 and 180 women were recruited, respectively. Results: The rs12589592 (G>A) polymorphism analysis showed 141 GG homozygotes (pelvic organ prolapse group: 53 [47.7%] and Control: 88 [49.2%] p=0.850); and 149 AA+GA: (pelvic organ prolapse group: 58 [52.3%]; Control: 91 [50.8%]; p=0.904). The distribution of genotypes did not follow the Hardy-Weinberg equilibrium conditions. Conclusion: There was no difference between groups regarding genotypes (rs12589592 G>A) frequency; however, the population characteristics prevent the analysis of the association between the genotype and the occurrence of prolapse.
Databáze:	MEDLINE
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