Sex-specific impact of obstructive sleep apnea on peripheral blood mononuclear cells.

Autor: Bock JM; Department of Cardiovascular Medicine, Mayo Clinic, Rochester, MN, 55901, USA., Vungarala S; Department of Cardiovascular Medicine, Mayo Clinic, Rochester, MN, 55901, USA., Sompalli S; Department of Cardiovascular Medicine, Mayo Clinic, Rochester, MN, 55901, USA., Singh P; Department of Cardiovascular Medicine, Mayo Clinic, Rochester, MN, 55901, USA.; Pennington Biomedical Research Center, Baton Rouge, LA, USA., Pavelko KD; Immunology, Mayo Clinic, Rochester, MN, USA., Kennedy RB; Immunology, Mayo Clinic, Rochester, MN, USA., Somers VK; Department of Cardiovascular Medicine, Mayo Clinic, Rochester, MN, 55901, USA. somers.virend@mayo.edu.
Jazyk: angličtina
Zdroj: Sleep & breathing = Schlaf & Atmung [Sleep Breath] 2024 Dec 04; Vol. 29 (1), pp. 44. Date of Electronic Publication: 2024 Dec 04.
DOI: 10.1007/s11325-024-03201-2
Abstrakt: Purpose: Experimental sleep disruption in healthy adults is more deleterious to immune function in females relative to males; however, it remains unknown if this translates to patients with obstructive sleep apnea (OSA). Thus, this study explored sex differences in peripheral blood mononuclear cells (PBMCs) from patients with untreated OSA.
Methods: Participants completed sleep studies to identify the presence of OSA via the apnea-hypopnea index (AHI). PBMCs were isolated, cryopreserved, and batch phenotyped via mass cytometry.
Results: Females with (n = 6, AHI = 25.9 ± 21.4 events/hr, age = 37 ± 14yrs, BMI = 30.5 ± 7.4 kg/m 2 ) and without (n = 9, AHI = 2.6 ± 1.6 events/hr, age = 35 ± 10yrs, BMI = 29.2 ± 6.3 kg/m 2 ) OSA were compared to males with (n = 7, AHI = 13.7 ± 8.5 events/hr, age = 33 ± 11yrs, BMI = 30.0 ± 4.8 kg/m 2 ) and without (n = 7, AHI = 2.6 ± 1.6 events/hr, age = 33 ± 10yrs, BMI = 28.9 ± 3.8 kg/m 2 ) OSA. No significant group-by-sex interactions were observed in CD3 T cells (p = 0.273), CD8 T cells (p = 0.656), B cells (p = 0.190), monocytes (p = 0.638), nor granulocytes (p = 0.267) expressed as a percent of their respective parent population. While the percentage of total NK cells did not differ between groups (group-by-sex p = 0.822), females with OSA had fewer CD57 - (42.4 ± 14.7 vs. 62.4 ± 10.4%) and more CD57 + (57.6 ± 14.7 vs. 37.6 ± 10.4%) NK cells than females without OSA (p < 0.050). No differences in CD57 - (53.6 ± 18.1 vs. 44.9 ± 16.8%) and CD57 + (46.4 ± 18.1 vs. 55.2 ± 19.8%) NK cells were observed between males (p = 0.283). Tregs were more prevalent in females with vs. females without OSA (2.17 ± 0.64 vs. 1.31 ± 0.41%, p = 0.006) with no difference between males (1.55 ± 0.50 vs. 1.71 ± 0.71%, p = 0.601).
Conclusions: Our data suggest that OSA increases the prevalence of cytotoxic NK cells and Tregs in females. The causes and downstream effects of these changes remain undetermined.
Competing Interests: Declarations. Ethical approval: All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent: Informed consent was obtained from all individual participants included in the study. Conflict of interest: JMB, SV, SS, PS, and KDP have no disclosures to report. RBK has consulted with Merck Laboratories and Sanofi-Pasteur, has received grant funding from ICW Ventures for preclinical studies on a peptide-based COVID-19 vaccine, and has patents related to vaccinia, influenza, SARS-CoV-2, measles, and zika peptide vaccines. VKS has consulted for Zoll, ApniMed, Huxley, Lilly, and Jazz Pharmaceuticals, and is on the Scientific Advisory Board for Sleep Number Corporation. These activities are not related to the work reported in this study, have been reviewed by the Mayo Clinic Conflict of Interest Review Board, and are conducted in compliance with Mayo Clinic Conflict of Interest policies. The data that support the findings of this study are not openly available due to reasons of sensitivity and are available from the corresponding author upon reasonable request. Data are located in controlled access data storage at Mayo Clinic.
(© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
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