Intraspecific variability of rice root knot nematodes across diverse agroecosystems for sustainable management.

Autor: Mondal S; Indian Statistical Institute, Giridih, Jharkhand, 815301, India.; Department of Plant Pathology, The Ohio State University, Columbus, OH, 43210, USA., Purohit A; Indian Statistical Institute, Giridih, Jharkhand, 815301, India.; EVA 4.0 Unit, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Prague, Czechia.; Department of Genetics, University of Calcutta, Kolkata, West Bengal, 700019, India., Hazra A; Department of Genetics, University of Calcutta, Kolkata, West Bengal, 700019, India.; Department of Botany, Victoria Institution (College), 78-B, A.P.C. Road, Kolkata, West-Bengal, 700009, India., Das S; Division of Plant Biology, Bose Institute, Centenary Campus, Kolkata, West Bengal, 700054, India., Chakrabarti M; School of Integrative Biological and Chemical Sciences, University of Texas Rio Grande Valley, Edinburg, TX, USA., Khan MR; Division of Nematology, Indian Agricultural Research Institute, New Delhi, 110012, India., Lopez-Nicora H; Department of Plant Pathology, The Ohio State University, Columbus, OH, 43210, USA., Chakraborti D; Department of Genetics, University of Calcutta, Kolkata, West Bengal, 700019, India., Mukherjee A; Indian Statistical Institute, Giridih, Jharkhand, 815301, India. abhi.mukh@yahoo.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Dec 03; Vol. 14 (1), pp. 30032. Date of Electronic Publication: 2024 Dec 03.
DOI: 10.1038/s41598-024-73980-x
Abstrakt: In the rice agroecosystems of Southeast Asia, rice root knot nematode (Meloidogyne graminicola) significantly impairs yield, representing a major species within the 'graminis-group' known for its morphological similarities with other root knot nematodes (RKNs). This study delves into the variations in reproductive potential, morphology, morphometrics, and genetic diversity among thirty RKN populations in rice across three distinct agroecological zones in Jharkhand, India. Despite notable differences in reproductive potential among the populations, morphological and morphometric correlations to reproductive potential were inconclusive. However, male and juvenile morphometrics were crucial for identifying intraspecific variability. Genetic analysis utilizing five molecular markers (ITS, 18 S rRNA, D2-D3 of 28 S, COX-I, and COX-II) affirmed the populations as M. graminicola, with ITS marker revealing significant intraspecific variability. Phylogenetic analysis underscored the close relationship between M. oryzae and M. graminicola, distinct from other mitotic RKN species. Low genetic distance and nucleotide diversity, coupled with high haplotype diversity, negative Tajima's D, and Fu's Fs of haplotype network analysis, suggested that all M. graminicola populations are expanding. These findings highlight the urgent need for comprehensive management strategies against M. graminicola, providing valuable insights for growers, extension officials, and plant breeders to develop targeted management approaches and resistance breeding programs.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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