| Autor: |
Khosravi M; 1Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; 2Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Khosravi F; 1Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Pouresmaeil O; 1Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; 2Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.; 3Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Aryan E; 1Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; 2Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Meshkat Z; 1Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; 2Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Safdari H; 1Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Farsiani H; 1Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; 2Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. |
| Abstrakt: |
Shigellosis, a diarrheal disease caused by Shigella species, is a significant public health concern, particularly in developing countries with inadequate sanitation systems. This study aimed to investigate the patterns of antibiotic resistance, ESBL and AmpC genes, integrons, and enterotoxin genes in Shigella species isolated from patients with gastroenteritis in Northeast Iran. This cross-sectional study was conducted between January 2017 and December 2019 at a tertiary care hospital in Northeast Iran. A total of 110 Shigella isolates were collected from stool samples of patients with gastroenteritis. The isolates were identified using conventional biochemical tests and confirmed by PCR. The highest resistance rates were detected for ampicillin (88.2%) and cotrimoxazole (84.5%). Altogether 64.5% of isolates exhibited multidrug resistance however, ESBL and AmpC phenotypes were detected in 34.54% and 1.81% of isolates, respectively. Interestingly, blaCTX-M-15 and blaTEM were detected in all ESBL-positive isolates but integron class 1, 2, and 3 were identified in 97.3%, 76.4%, and 59.1% of isolates, respectively. The sen gene was present in 72.7% of the isolates. In this study CTX-M-15 production was detected in 31 strains of Shigella sonnei and in 7 strains of Shigella flexneri. The high prevalence of multidrug-resistant Shigella isolates is concerning and shows the need for continuous monitoring and rational use of antibiotics. |
