Exploring aortic stiffness in aging mice: a comprehensive methodological overview.

Autor: Vanalderwiert L; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France., Henry A; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France., de Souza E Silva JM; Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Halle (Saale) 06120, Germany., Carvajal-Berrio D; Department for Medical Technologies and Regenerative Medicine, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Tübingen 72076, Germany., Debelle L; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France., Wahart A; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France., Marzi J; Department for Medical Technologies and Regenerative Medicine, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Tübingen 72076, Germany.; NMI Natural and Medical Sciences Institute, Reutlingen 72770, Germany., Schenke-Layland K; Department for Medical Technologies and Regenerative Medicine, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Tübingen 72076, Germany.; NMI Natural and Medical Sciences Institute, Reutlingen 72770, Germany.; Department of Medicine/Cardiology, Cardiovascular Research Laboratories, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA., Faury G; INSERM, CHU Grenoble Alpes, University of Grenoble Alpes, Grenoble 38000, France., Six I; Research Unit 7517, Pathophysiological Mechanisms and Consequences of Cardiovascular Calcifications (MP3CV), University of Picardie Jules Verne, Amiens, France., Schmelzer CEH; Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Halle (Saale) 06120, Germany.; Institute of Pharmacy, Faculty of Natural Sciences I, Martin Luther University Halle-Wittenberg, Halle (Saale) 06120, Germany., Brinckmann J; Institute of Virology and Cell Biology, University of Lübeck, Lübeck, Germany.; Department of Dermatology, University of Lübeck, Lübeck, Germany., Steenbock H; Institute of Virology and Cell Biology, University of Lübeck, Lübeck, Germany., Almagro S; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France., Delacoux F; International coordinator of The Exact and Natural Faculty of Reims, University of Reims Champagne-Ardenne, Reims 51100, France., Jaisson S; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France.; Department of Biochemistry, Hospital of Reims, Reims, France., Gillery P; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France.; Department of Biochemistry, Hospital of Reims, Reims, France., Maurice P; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France., Sartelet H; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France., Bennasroune A; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France., Duca L; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France., Romier B; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France., Blaise S; UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France.
Jazyk: angličtina
Zdroj: Aging [Aging (Albany NY)] 2024 Dec 02; Vol. null. Date of Electronic Publication: 2024 Dec 02.
DOI: 10.18632/aging.206168
Abstrakt: Stiffening of the vascular network is associated with the early stages of vascular aging, leading to cardiovascular disorders (hypertension), renal failures, or neurodegenerative diseases (Alzheimer's). Unfortunately, many people remain undiagnosed because diagnostic methods are either unsuitable for a large population or unfamiliar to clinicians which favor the hypertension evaluation. In preclinical research, stiffness studies are often partially conducted. We think that the evaluation of aortic stiffness is essential as it would improve our understanding of aging diseases progression. We propose here a systematic method using decision trees in a multi-scale and multimodal approaches. Our method was evaluated by analyzing the aortic situation in old and young mice. We demonstrate that both the endothelial and smooth muscle cells exhibit pronounced functional alterations in favor of constriction. Additionally, there is significant remodeling of the extracellular matrix, leading to a drastic degradation of elastic fibers and the accumulation of collagen in the aortic wall. This series of changes contributes to the development of vascular rigidity, a preliminary stage of arterial hypertension. Our results suggest that our method should improve preclinical understanding and encourage clinicians to equip themselves with tools for assessing vascular function, as it is an essential issue for preventing numerous pathologies.
Databáze: MEDLINE