Genetic Diversity and Population Dynamics of Clinostomum spp. Using Comprehensive Bioinformatics Approaches.

Autor: Islam SI; Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand., Hamad MH; Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.; Infectious Diseases, Department of Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt., Jitsamai W; Department of Parasitology and Entomology, Faculty of Public Health, Mahidol University, Bangkok, Thailand., Rodkhum C; Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand., Taweethavonsawat P; Parasitology Unit, Department of Pathology and Biomarkers in Animal Parasitology Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Jazyk: angličtina
Zdroj: Veterinary medicine international [Vet Med Int] 2024 Nov 23; Vol. 2024, pp. 6924523. Date of Electronic Publication: 2024 Nov 23 (Print Publication: 2024).
DOI: 10.1155/vmi/6924523
Abstrakt: Clinostomum species, a parasitic pathogen of freshwater fish, is widely distributed and infects various host species. Recently, the pathological effect due to Clinostomum metacercarial infection was described in aquaculture in Thailand; however, the global genetic diversity and population structure of this species have not been studied yet. Therefore, this study aimed to provide a detailed description of genetic diversity and population dynamics of the digenean Clinostomum isolated from Trichopodus pectoralis with globally recorded Clinostomum species. The species was characterized molecularly by analyzing 18S rDNA and inter-transcribed spacer biomarker genes (ITS1 and ITS2). A BLAST search discovered that the 18S rDNA and ITS sequence had a 100% sequence similarity with Clinostomum piscidium isolated from India and Thailand. A comprehensive analysis revealed the presence of 12 distinct haplotypes among the Clinostomum populations. This study suggests that distinct patterns of genetic variation were identified by analyzing molecular variance, pairwise Fst, and employing structure analysis. It was observed that a gradient of genetic variation exists within continents, characterized by higher levels within different groups and lower levels of genetic differentiation. Additionally, a notable presence of mixed haplotypes was observed. The results of neutrality testing suggest that there has been a significant expansion in the populations of Clinostomum in India, America, and Kenya. The discoveries from this study will provide a valuable contribution to comprehending the genetics and evolution of Clinostomum species. Furthermore, key findings will be essential in developing efficient management approaches to prevent and control this parasite.
Competing Interests: The authors declare no conflicts of interest.
(Copyright © 2024 Sk Injamamul Islam et al.)
Databáze: MEDLINE
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