Development of a Myogenin minimal promoter-based system for visualizing the degree of myogenic differentiation.

Autor: Fumoto Y; Department of Molecular Biology, Graduate School of Medicine, Hokkaido University, Japan. Electronic address: moon.yf.6411@gmail.com., Takada S; Department of Sports Education, Faculty of Lifelong Sport, Hokusho University, Japan., Onodera Y; Department of Molecular and Cellular Dynamics Research, Graduate School of Biomedical Science and Engineering, Hokkaido University, Japan., Hatakeyama S; Department of Biochemistry, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Japan., Oikawa T; Department of Molecular Biology, Graduate School of Medicine, Hokkaido University, Japan. Electronic address: oikawa_tsukasa@med.hokudai.ac.jp.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Dec 31; Vol. 741, pp. 151091. Date of Electronic Publication: 2024 Nov 28.
DOI: 10.1016/j.bbrc.2024.151091
Abstrakt: Myogenic differentiation plays a fundamental role in myogenesis during development and in muscle regeneration. Sequential expression of myogenic regulatory factors (MRFs) including myogenin in the progenitor cells triggers the expression of effector proteins such as myosin heavy chain (MHC), leading to the terminal muscle differentiation. Although we have a snapshot-like understanding of molecules at each stage of the differentiation, how these molecules are interrelated in the continuum of myogenic differentiation remains poorly understood. In this study, we analyzed the dynamics of the minimal Myogenin promoter activity in live myoblasts. With the development of a new co-expression analysis method, we were able to reveal in detail the relationship between this Myogenin promoter activity and the expression of endogenous myogenin or MHC, as differentiation markers. Consequently, we found that our visualization system of myogenic differentiation is suitable for monitoring the transition from myoblasts to myotubes, in which the Myogenin promoter activity quantitatively represents the degree of myogenic differentiation. Thus, this system allows simultaneous observation of the degree of myoblast differentiation in relation to other molecules, which would contribute to deepening our understanding of myogenic differentiation as a continuous process.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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