Intracellular polarization of RNAs and proteins in the human small intestinal epithelium.

Autor: Novoselsky R; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Harnik Y; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Yakubovsky O; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.; Department of General Surgery and Transplantation, Sheba Medical Center, Ramat Gan, Israel.; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Katina C; The De Botton Protein Profiling, The Nancy and Stephen Grand Israel and Health Sciences National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel., Levin Y; The De Botton Protein Profiling, The Nancy and Stephen Grand Israel and Health Sciences National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel., Bahar Halpern K; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel., Pencovich N; Department of General Surgery and Transplantation, Sheba Medical Center, Ramat Gan, Israel.; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Nachmany I; Department of General Surgery and Transplantation, Sheba Medical Center, Ramat Gan, Israel.; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Itzkovitz S; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Jazyk: angličtina
Zdroj: PLoS biology [PLoS Biol] 2024 Dec 02; Vol. 22 (12), pp. e3002942. Date of Electronic Publication: 2024 Dec 02 (Print Publication: 2024).
DOI: 10.1371/journal.pbio.3002942
Abstrakt: The intestinal epithelium is a polarized monolayer of cells, with an apical side facing the lumen and a basal side facing the blood stream. In mice, both proteins and mRNAs have been shown to exhibit global basal-apical polarization; however, polarization in the human intestine has not been systematically explored. Here, we employed laser-capture microdissection to isolate apical and basal epithelial segments from intestinal tissues of 8 individuals and performed RNA sequencing and mass-spectrometry proteomics. We find a substantial polarization of mRNA molecules that largely overlaps polarization patterns observed in mice. This mRNA polarization remains consistent across different zones of the intestinal villi and is generally correlated with the polarization of proteins. Our protein analysis exposes streamlined intracellular nutrient transport and processing and reveals that mitochondria and ribosomes are less polarized in humans compared to mice. Our study provides a resource for understanding human intestinal epithelial biology.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Novoselsky et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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