| Autor: |
Lee DC; Department of Biology, Boston College, Massachusetts, USA., Ta L; Department of Biology, Boston College, Massachusetts, USA., Mukherjee P; Department of Biology, Boston College, Massachusetts, USA., Duraj T; Department of Biology, Boston College, Massachusetts, USA., Domin M; Mass Spectrometry Center, Chemistry Department, Boston College, Massachusetts, USA., Greenwood B; BPGbio, Framingham, Massachusetts, USA., Karmacharya S; BPGbio, Framingham, Massachusetts, USA., Narain NR; BPGbio, Framingham, Massachusetts, USA., Kiebish M; BPGbio, Framingham, Massachusetts, USA., Chinopoulos C; Department of Biochemistry, Semmelweis University, Budapest, Hungary., Seyfried TN; Department of Biology, Boston College, Massachusetts, USA. |
| Abstrakt: |
Energy is necessary for tumor cell viability and growth. Aerobic glucose-driven lactic acid fermentation is a common metabolic phenotype seen in most cancers including malignant gliomas. This metabolic phenotype is linked to abnormalities in mitochondrial structure and function. A luciferin-luciferase bioluminescence ATP assay was used to measure the influence of amino acids, glucose, and oxygen on ATP content and viability in mouse (VM-M3 and CT-2A) and human (U-87MG) glioma cells that differed in cell biology, genetic background, and species origin. Oxygen consumption was measured using the Resipher system. Extracellular lactate and succinate were measured as end products of the glycolysis and glutaminolysis pathways, respectively. The results showed that: (1) glutamine was a source of ATP content irrespective of oxygen. No other amino acid could replace glutamine in sustaining ATP content and viability; (2) ATP content persisted in the absence of glucose and under hypoxia, ruling out substantial contribution through either glycolysis or oxidative phosphorylation (OxPhos) under these conditions; (3) Mitochondrial complex IV inhibition showed that oxygen consumption was not an accurate measure for ATP production through OxPhos. The glutaminase inhibitor, 6-diazo-5-oxo-L-norleucine (DON), reduced ATP content and succinate export in cells grown in glutamine. The data suggests that mitochondrial substrate level phosphorylation in the glutamine-driven glutaminolysis pathway contributes to ATP content in these glioma cells. A new model is presented highlighting the synergistic interaction between the high-throughput glycolysis and glutaminolysis pathways that drive malignant glioma growth and maintain ATP content through the aerobic fermentation of both glucose and glutamine. |
