| Autor: |
Cummings CB; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Catania SS; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Ednacot EMQ; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Kinsella-Johnson AJ; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Meeds CE; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Reynolds JW; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Sanderson AE; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Johnson RA; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University., Watts KR; Department of Chemistry and Biochemistry, California Polytechnic State University; Center for Applications in Biotechnology, California Polytechnic State University; krwatts@calpoly.edu. |
| Abstrakt: |
The continuous advancement of molecular biology techniques requires that molecular biology curricula are regularly refined to effectively prepare students to enter the workforce with modern competencies. In particular, the emergence of Gibson Assembly, a highly customizable and adaptive molecular cloning technique, has advanced the landscape of molecular cloning in numerous research environments. Thus, we created a Gibson Assembly cloning module for deployment in a molecular biology laboratory course at California Polytechnic State University, San Luis Obispo and evaluated student learning outcomes from the module. Over three iterations of the course, students participated in an experiment-based independent project that involved cloning three unique plasmid libraries to support research projects in natural products biosynthesis. Students were given pre- and post-questionnaires to evaluate their understanding of molecular cloning and their confidence in molecular biology terms and techniques. Students' responses showed a significant increase in both learning molecular cloning concepts and in self-reported confidence with molecular cloning terms and techniques. This module framework can be generalized to teach Gibson Assembly for various applications, providing instructors with a toolkit for teaching an adaptable and emergent cloning technology while advancing their research projects. |
