Synthesis, Biological Evaluation and in Silico Studies of Novel Urea/Thiourea Derivatives of Lenalidomide.

Autor: Tok F; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Marmara University, Istanbul, Turkiye., Abas Bİ; Department of Biochemistry, School of Medicine, Aydın Adnan Menderes University, Aydın, Turkiye., Başoğlu F; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, European University of Lefke, Northern Cyprus, Turkiye., Çevik Ö; Department of Biochemistry, School of Medicine, Aydın Adnan Menderes University, Aydın, Turkiye., Karakuş S; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Istanbul Aydin University, Istanbul, Turkiye., Ece A; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Biruni University, Istanbul, Turkiye.
Jazyk: angličtina
Zdroj: Journal of biochemical and molecular toxicology [J Biochem Mol Toxicol] 2024 Dec; Vol. 38 (12), pp. e70079.
DOI: 10.1002/jbt.70079
Abstrakt: Designing new compounds from existing chemotherapeutic drugs to enhance inhibitory effects on tumor cells while overcoming multidrug resistance is one of the important strategies for new drug discovery in medicinal chemistry. A new series of urea and thiourea derivatives based on Lenalidomide as potential anticancer agents have been designed and synthesized. In vitro anticancer activity assay against Caki cancer cells and HUVEC endothelial cells revealed that 1-(4-methylphenyl)-3-[2-(2,6-dioxopiperidin-3-yl)-1-oxoisoindolin-4-yl]urea (11) exhibited the highest anticancer activity and selectivity in the series with IC 50 values of 9.88 and 179.03 µM, respectively. Among the compounds, 11 showed significant HDAC1 inhibiton of 68.02 ± 2.44% at 10 µM concentration. TGF-β, Bax, Bcl-2 protein levels and scratch assay were analyzed in Caki cells. As a result, compound 11 induced apoptosis in Caki cells. In this study, it has been demonstrated that compound 11 can be a lead compound for further detailed investigation in renal cancer treatment. Through molecular docking studies, it was determined that the most active compound, 11, forms stable interactions with key residues in the enzyme's active site, particularly engaging in hydrogen bonds with GLY149 and coordinating with the zinc ion in the HDAC1 active site. These interactions are crucial for the observed inhibitory activity. Molecular dynamics simulation revealed the binding event of the most active compound with class I histone deacetylase and the stability of the complex in a biological environment.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
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