Moldable Alginate/Hydroxyapatite Hydrogel Loaded with Metformin Enhanced Regeneration of the Rabbit Mandibular Defects.

Autor: Nabavizadeh SS; Department of Otolaryngology, Otolaryngology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran., Shadi M; Anatomy Department, Shiraz Medical School, Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran., Khorraminejad-Shirazi M; Student Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.; Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.; Cell and Molecular Medicine Student Research Group, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Daneshi S; Tissue Engineering Lab, Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Tavanafar S; Department of Oral and Maxillofacial Surgery, School of Dentistry, Birjand University of Medical Sciences, Birjand, Iran., Naseri R; Student Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Abbaspour A; Student Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Alaei-Jahromi K; Student Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.; Radiology Department, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran., Talaei-Khozani T; Anatomy Department, Shiraz Medical School, Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.; Tissue Engineering Lab, Dept. of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
Jazyk: angličtina
Zdroj: Journal of maxillofacial and oral surgery [J Maxillofac Oral Surg] 2024 Dec; Vol. 23 (6), pp. 1391-1404. Date of Electronic Publication: 2024 Feb 04.
DOI: 10.1007/s12663-023-02094-0
Abstrakt: Background: Moldable hydrogel-based techniques loaded with osteoinductive agents such as metformin have become a promising field for reconstructing critical-sized bone defects, particularly in those with irregular shapes. Here, we used metformin incorporated in an alginate/hydroxyapatite hydrogel to accelerate the repair of the rabbit critical-sized mandibular defect.
Methods: Cytotoxicity and osteoinduction of the metformin-loaded alginate/hydroxyapatite hydrogel were evaluated by culturing the osteosarcoma cell line (MG63). Moreover, in vivo bone formation was assessed in a rabbit bone defect model using computed tomography and histomorphometric analysis to compare the effects of alginate/hydroxyapatite hydrogel with or without metformin.
Results: The data showed that the scaffolds were not cytotoxic and enhanced osteogenic characteristics of the cells, as manifested by augmented alkaline phosphatase activity and calcium deposition . In vivo studies indicated that all the treated groups exhibited more osteogenesis with a significant increase in bone-specific cell population and less residual scaffold remnant at the defect sites compared with the control group, which was significantly prominent in the group treated with alginate/hydroxyapatite/metformin. Moreover, computed tomography scan analysis also confirmed better bone filling in all the treated groups, especially in the defects treated with alginate/hydroxyapatite/metformin hydrogel.
Conclusions: Both In vitro and in vivo experiments revealed that locally loaded metformin with the easy size- and shape-adapted alginate/hydroxyapatite hydrogel has proper biocompatibility and osteogenesis properties. Moreover, our study highlighted the synergistic effect of metformin and hydroxyapatite on osteogenesis.
Competing Interests: Conflict of interestThe authors have no conflict of interest to disclose.
(© The Association of Oral and Maxillofacial Surgeons of India 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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