Rapid and Safe Neutralization Assay for Circulating H5N1 Influenza Virus in Dairy Cows.

Autor: Miyakawa K; Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Tokyo, Japan.; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan.; Department of Microbiology, Yokohama City University School of Medicine, Kanagawa, Japan., Ota M; Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Tokyo, Japan.; Department of Microbiology, Yokohama City University School of Medicine, Kanagawa, Japan., Sano K; Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Tokyo, Japan., Momose F; Center for Emergency Preparedness and Response, National Institute of Infectious Diseases, Tokyo, Japan., Okura T; Department of Virology III, National Institute of Infectious Diseases, Tokyo, Japan., Kishida N; Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Tokyo, Japan., Arita T; Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Tokyo, Japan., Suzuki Y; Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Tokyo, Japan., Shirakura M; Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Tokyo, Japan., Asanuma H; Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Tokyo, Japan., Watanabe S; Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Tokyo, Japan., Ryo A; Department of Microbiology, Yokohama City University School of Medicine, Kanagawa, Japan.; Department of Virology III, National Institute of Infectious Diseases, Tokyo, Japan., Hasegawa H; Research Center for Influenza and Respiratory Viruses, National Institute of Infectious Diseases, Tokyo, Japan.
Jazyk: angličtina
Zdroj: Influenza and other respiratory viruses [Influenza Other Respir Viruses] 2024 Dec; Vol. 18 (12), pp. e70048.
DOI: 10.1111/irv.70048
Abstrakt: Rapid and safe neutralization assays are required for highly pathogenic avian influenza viruses, including a clade 2.3.4.4b H5N1 subtype recently found in cows. Here, we report a neutralization assay using luminescent virus-like particles. This assay has lower biosafety requirements and provides a larger dynamic range than conventional methods. We applied this technique to evaluate the cross-reactivity of neutralizing antibodies induced by clade 2.3.4.4b candidate vaccine viruses (CVVs) with the cow-derived H5N1 virus. Our findings indicate that these CVVs share antigenic characteristics with the cow-derived H5N1 virus, suggesting the potential efficacy of vaccines developed using these CVVs.
(© 2024 The Author(s). Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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