Investigation of natural infection of BALB C mice by Bartonella henselae.

Autor:	Santos LSD; Universidade Estadual de Campinas (UNICAMP), Faculdade de Ciências Médicas (FCM), Laboratório de Pesquisa Aplicada Dermatologia e Infecção por Bartonella, Campinas, SP, Brazil., Martins SAS; Universidade Estadual de Campinas (UNICAMP), Faculdade de Ciências Médicas (FCM), Laboratório de Pesquisa Aplicada Dermatologia e Infecção por Bartonella, Campinas, SP, Brazil., Scheffer FR; Universidade Estadual de Campinas (UNICAMP), Faculdade de Ciências Médicas (FCM), Laboratório de Pesquisa Aplicada Dermatologia e Infecção por Bartonella, Campinas, SP, Brazil., Maekawa AS; Universidade Estadual de Campinas (UNICAMP), Faculdade de Ciências Médicas (FCM), Laboratório de Pesquisa Aplicada Dermatologia e Infecção por Bartonella, Campinas, SP, Brazil; Memorial University of Newfoundland, Faculty of Medicine - Endocrinology, St. John's, Newfoundland and Labrador, Canada., Silva RP; Universidade Estadual de Campinas (UNICAMP), Faculdade de Ciências Médicas (FCM), Laboratório de Pesquisa Aplicada Dermatologia e Infecção por Bartonella, Campinas, SP, Brazil., de Araújo GR; Universidade Estadual de Campinas (UNICAMP), Faculdade de Ciências Médicas (FCM), Laboratório de Pesquisa Aplicada Dermatologia e Infecção por Bartonella, Campinas, SP, Brazil., Velho PENF; Universidade Estadual de Campinas (UNICAMP), Faculdade de Ciências Médicas (FCM), Laboratório de Pesquisa Aplicada Dermatologia e Infecção por Bartonella, Campinas, SP, Brazil; Universidade Estadual de Campinas (UNICAMP), Departamento de Medicina, Divisão de Dermatologia, Campinas, SP, Brazil. Electronic address: pvelho@unicamp.br., Drummond MR; Universidade Estadual de Campinas (UNICAMP), Faculdade de Ciências Médicas (FCM), Laboratório de Pesquisa Aplicada Dermatologia e Infecção por Bartonella, Campinas, SP, Brazil.
Jazyk:	angličtina
Zdroj:	The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases [Braz J Infect Dis] 2024 Nov 29; Vol. 29 (1), pp. 104483. Date of Electronic Publication: 2024 Nov 29.
DOI:	10.1016/j.bjid.2024.104483
Abstrakt:	Specific Pathogen-Free (SPF) animals are bred and maintained to exclude pathogens associated with significant morbidity or mortality, which may pose a risk to research replicability. The BALB/c strain is distributed globally and is among the most commonly used inbred strains in immunology and infectious disease research. Despite being a widely distributed bacterium that causes chronic infection, Bartonella henselae infection has not been investigated in any protocol that characterizes SPF animals. The objective of this study was to investigate the potential natural infection of laboratory animals of the BALB/c lineage by B. henselae. To achieve this, ten immunocompetent BALB/c mice were obtained directly from the bioterium and euthanized for collection of samples, including blood, skin, spleen, liver, heart, eye, kidney, intestine, esophagus, and brain. DNA was extracted using a commercial kit and tested via nested PCR for the ftsZ gene, as well as conventional PCR and qualitative real-time PCR using Sybr® Green for the citrate synthase gene (gltA), all specific reactions for B. henselae. All animals showed detection of B. henselae DNA in at least two different reactions in different tissues. The sequenced amplicons showed 100 % similarity to B. henselae. The use of mice infected by B. henselae in experiments is undesirable, as the bacteria can affect several aspects of the animal's physiology and consequently influence the results of the project, especially when subjected to immunosuppression. More studies are needed to understand and confirm the natural infection in experimental animals by Bartonella spp.. To date, no additional published reports of contamination of experimental animals by these bacteria have been identified. Competing Interests: Conflicts of interest The authors declare no conflicts of interest. (Copyright © 2024 Sociedade Brasileira de Infectologia. Published by Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
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