Embryology outcomes of a device-based sperm separation technique compared to density gradient centrifugation using thawed spermatozoa-a sibling donor oocyte study.

Autor: Gavriil E; Department of Embryology and Andrology, Newlife IVF Greece, 171 Ethnikis Antistaseos Str, Kalamaria, 55134, Thessaloniki, Greece. egavriil@newlife-ivf.gr., Desli A; Department of Embryology and Andrology, Newlife IVF Greece, 171 Ethnikis Antistaseos Str, Kalamaria, 55134, Thessaloniki, Greece., Geladaris V; Department of Embryology and Andrology, Newlife IVF Greece, 171 Ethnikis Antistaseos Str, Kalamaria, 55134, Thessaloniki, Greece., Kachpani E; Department of Embryology and Andrology, Newlife IVF Greece, 171 Ethnikis Antistaseos Str, Kalamaria, 55134, Thessaloniki, Greece., Neofytou E; Department of Embryology and Andrology, Newlife IVF Greece, 171 Ethnikis Antistaseos Str, Kalamaria, 55134, Thessaloniki, Greece., Tatsi P; Department of Embryology and Andrology, Newlife IVF Greece, 171 Ethnikis Antistaseos Str, Kalamaria, 55134, Thessaloniki, Greece., Dovas D; Department of Obstetrics and Gynecology, Newlife IVF Greece, 171 Ethnikis Antistaseos Str, Kalamaria, 55134, Thessaloniki, Greece.
Jazyk: angličtina
Zdroj: Journal of assisted reproduction and genetics [J Assist Reprod Genet] 2024 Nov 30. Date of Electronic Publication: 2024 Nov 30.
DOI: 10.1007/s10815-024-03336-x
Abstrakt: Objective: To evaluate whether the ZyMōt™ Multi 850 μl sperm separation device (SSD) effectively recovers motile spermatozoa from cryopreserved ejaculates and compare its effect on key embryology outcomes including fertilization, cleavage stage, and total and top-quality blastocyst formation rates to the traditional Density Gradient Centrifugation (DGC) method.
Methods: In this prospective, single-center, controlled study, we used fresh sibling donor oocytes and non-donor cryopreserved ejaculates. In total, 150 couples participated in this study. At least eight MII donor oocytes were allocated to each couple split into two arms. One arm underwent ICSI with the control DGC-processed sample, and the other arm processed with SSD.
Results: No significant difference on fertilization and cleavage stage embryo rates was observed between the two techniques. We observed a significant increase in the percentage of total (SSD: 74.03 ± 23.47% vs. DGC: 67.86 ± 23.92%; p = 0.016) and top-quality (SSD: 66.38 ± 24.94% vs. DGC: 60.98 ± 24.40%; p = 0.035) blastocysts formed post-SSD processing. Sub-analysis showed that this increase remained significant for the WHO-normal group (n = 118), but not for the WHO-abnormal group (n = 32).
Conclusion: The SSD was successfully applied in all 150 cases, providing adequate numbers of spermatozoa to undergo ICSI. Additionally, SSD significantly improved blastocyst development rates; however, this was of limited clinical impact considering the minor improvement on the average number of top-quality blastocysts. It can be hypothesized that this positive contribution may be stronger and clinically significant when a larger number of oocytes is used or in homologous oocyte ICSI cycles, where the repair mechanisms of the oocytes may insufficient for promoting healthy embryo development.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests. I hereby declare that all information is true and accurate and I acknowledge that any falsification, or concealment of material fact may subject me to administrative, civil, or criminal liability. I also declare that all data is original and have not been previously published or submitted anywhere.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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