Bee pollen from bracatinga (Mimosa scabrella): Effects of gastrointestinal digestion and epithelial transport in vitro on phenolic profile and bioactivities.

Autor: Saliba ASMC; Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brazil., Sartori AGO; Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba, SP, Brazil; Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP, Brazil. Electronic address: alangosartori@usp.br., Rosalen PL; Faculdade de Odontologia, Universidade Estadual de Campinas, Piracicaba, SP, Brazil; Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, MG, Brazil., Lazarini JG; Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba, SP, Brazil., do Amaral JEPG; Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brazil., da Luz CFP; Instituto de Pesquisas Ambientais, São Paulo, SP, Brazil., Martarello NS; Instituto de Pesquisas Ambientais, São Paulo, SP, Brazil., Torres LCR; Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brazil., de Souza LM; Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba, SP, Brazil., de Alencar SM; Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brazil; Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba, SP, Brazil. Electronic address: smalencar@usp.br.
Jazyk: angličtina
Zdroj: Food research international (Ottawa, Ont.) [Food Res Int] 2024 Nov; Vol. 196, pp. 115142. Date of Electronic Publication: 2024 Sep 26.
DOI: 10.1016/j.foodres.2024.115142
Abstrakt: The main objective of the present work was to assess the phenolic profile of bracatinga (Mimosa scabrella) bee pollen, and its antioxidant and anti-inflammatory activities after gastrointestinal digestion in vitro and epithelial transport in a Caco-2 cell monolayer model. The botanical origin of bee pollen was confirmed by optical microscopy and scanning electron microscopy. As major results, 34 phenolic compounds (13 phenylamides, 14 flavonols, and 7 flavanones) were tentatively identified in the extract of bracatinga bee pollen by HPLC-ESI-QTOF-MS. The aglycone forms of quercetin and p-coumaric acid were identified only after digestion, indicating the breakage of flavonols and phenylamides, respectively. These compounds may have contributed to the decrease in NF-κΒ activation up to 54% and in the release of TNF-α and CXCL2/MIP-2 by 26% and 21%, respectively, in raw 264.7 murine macrophages activated with microbial lipopolysaccharide and treated with the digested fraction. Among all tentatively identified phenolic compounds, five of them were found in the basolateral fraction. These compounds, represented by four aglycone flavonoids (quercetin, kaempferol, naringenin, and herbacetin methyl ether) and a phenolic acid (p-coumaric acid) may be responsible for its outstanding antioxidant activity in Caco-2 cells, as well as for its remaining capacity in mitigating CXCL2/MIP-2 release after transport through the Caco-2 cell monolayer, as an intestinal barrier model. Therefore, our work sheds light on the phenolic profile and bioactivities of an interesting functional food produced by bees throughout a simulated gastrointestinal system.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Ana Sofia Martelli Chaib Saliba reports financial support was provided by National Council for Scientific and Technological Development. Severino Matias de Alencar reports financial support was provided by Coordination of Higher Education Personnel Improvement. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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