Sperm acrosome-associated 1 (SPACA1) mRNA and protein molecules deficiency indicate low fertility and semen quality of Bali bulls (Bos sondaicus).

Autor: Agil M; Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, West Java, Indonesia. Electronic address: rhinogil@apps.ipb.ac.id., Pardede BP; Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, West Java, Indonesia; Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia. Electronic address: berlinpardede@gmail.com., Purwantara B; Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, West Java, Indonesia. Electronic address: purwantara@apps.ipb.ac.id., Arifiantini RI; Division of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, West Java, Indonesia. Electronic address: arifiantini@apps.ipb.ac.id., Hasbi H; Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar, South Sulawesi, Indonesia. Electronic address: hasbi_fapetunhas@yahoo.com., Sonjaya H; Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Makassar, South Sulawesi, Indonesia. Electronic address: sonjayaherry@gmail.com., Said S; Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia. Electronic address: syah006@brin.go.id., Suyadi S; Faculty of Animal Science, Brawijaya University, Malang, East Java, Indonesia. Electronic address: suyadi@ub.ac.id., Septian WA; Faculty of Animal Science, Brawijaya University, Malang, East Java, Indonesia. Electronic address: wike.andre@ub.ac.id., Nugraha CD; Research Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia. Electronic address: chai011@brin.go.id., Putri RF; Faculty of Animal Science, Brawijaya University, Malang, East Java, Indonesia. Electronic address: rafikafp@ub.ac.id., Ardianto A; Faculty of Animal Science, Brawijaya University, Malang, East Java, Indonesia. Electronic address: ari.ard13@ub.ac.id., Iskandar H; Research Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia. Electronic address: hikm008@brin.go.id., Pamungkas FA; Research Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Bogor, West Java, Indonesia. Electronic address: fitr046@brin.go.id., Memili E; Cooperative Agriculture Research Center, College of Agriculture, Food and Natural Resources, Prairie View, A&M University, Prairie View, TX, United States. Electronic address: ermemili@pvamu.edu.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2025 Feb; Vol. 233, pp. 80-87. Date of Electronic Publication: 2024 Nov 17.
DOI: 10.1016/j.theriogenology.2024.11.009
Abstrakt: Molecular-based biomarkers are believed to be more accurate in determining bulls' fertility and sperm's various fertility characteristics. The sperm acrosome-associated 1 (SPACA1) molecule, located in the anterior acrosomal and equatorial segments of the sperm head, is thought to be related to the function of binding and fusion between sperm and oocyte. This study aims to analyze the association of SPACA1 mRNA and protein with the fertility rate and semen quality of Bali bulls (Bos sondaicus) and assess its potential as a potential molecular biomarker determining bull fertility and sperm quality. Frozen semen from 20 Bali bulls was used in the research, which was then divided into two groups: high (HF) and low fertility (LF). SPACA1 mRNA abundance was analyzed using qRT-PCR, and SPACA1 protein abundance was analyzed using EIA. The semen quality parameters analyzed were motility (CASA), plasma membrane integrity (HOS test), sperm head morphology abnormalities (William staining), sperm viability (SYBR 14-PI), sperm capacitation (CTC assay), and sperm acrosome integrity (FITC-PNA). The results showed that there was a significant difference (P < 0.05) between high fertility (HF) and low fertility (LF) bulls, both in the abundance of SPACA1 at the mRNA, protein, and semen quality levels. Overall, the correlation test results showed a close relationship (P < 0.01) between the abundance of SPACA1 mRNA and protein with field fertility and various semen quality parameters tested in the study. A low level of SPACA1 molecules indicates low fertility and semen quality in Bali bulls. The SPACA1 molecule has the potential to be developed through further research to become a biomarker for determining fertility and semen quality in bulls.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: