Targeted isolation of lignans and triterpenoids from kadsura coccinea by molecular networking and anti-RA-FLS activity.

Autor: Liu SQ; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China., Xie QL; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China., Deng YS; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China., Liang L; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China., Yuan HW; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China., Li B; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China., Yu HH; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China., Tian X; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China., Qiu YX; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China., Paulin Kemayou Mouthe G; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China., Shehla N; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China., Zhang Y; Shenzhen Futian District Maternal and Child Health Hospital, Shenzhen, 518016, China., Cai ZB; Shenzhen Futian District Maternal and Child Health Hospital, Shenzhen, 518016, China., Wang W; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China. Electronic address: wangwei402@hotmail.com., Yang YP; TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China. Electronic address: yangyupei24@163.com.
Jazyk: angličtina
Zdroj: Phytochemistry [Phytochemistry] 2024 Nov 28; Vol. 231, pp. 114341. Date of Electronic Publication: 2024 Nov 28.
DOI: 10.1016/j.phytochem.2024.114341
Abstrakt: In this study, six compounds (four triterpenoids named heilaohutriterpenes A-D and two lignans name heilaohusuins F and G) together with 21 known compounds were isolated from roots of Kadsura coccinea (Lem.) A. C. Smith guided by molecular networking. Their structures were determined using a combination of HR-ESI-MS, 1D, 2D-NMR anatysis, NMR calculation, and electronic circular dichroism (ECD) calculations. Moreover, the ability of the isolated compounds to inhibit the proliferation of rheumatoid arthritis-fibroblastoid synovial (RA-FLS) cells was evaluated in vitro. Heilaohutriterpene B (2), heilaohutriterpene D (4), coccinone B (7), and kadsuralignan H (24) demonstrated significant inhibitory activities against RA-FLS cells, with IC 50 values of 9.57 ± 0.84, 16.22 ± 1.71, 3.08 ± 1.59, and 19.09 ± 2.42 μM, respectively. Meanwhile, western blotting analysis revealed that compound 2 down-regulated the level of P-NF-κB p65 and up-regulated that of Bax and IκBα. These results collectively suggest that compound 2 promoted the apoptosis of RA-FLS cells by inhibiting the NF-κB pathway. Taken together, this study contributed to the structural diversity of compounds derived from K. coccinea and lays a basis for further anti-RA-related studies.
Competing Interests: Declaration of competing interest On behalf of, and having obtained permission from all the authors, I declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled. I testify to the accuracy of the above on behalf of all the authors.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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