Catalase-like activity of perylene diimide based radical anion: Chromogenic substrate for achieving glucose sensing.

Autor: Kaur R; Department of Chemistry, UGC Centre for Advanced Studies-II, Guru Nanak Dev University, Amritsar 143001, Pb., India., Singh P; Department of Chemistry, UGC Centre for Advanced Studies-II, Guru Nanak Dev University, Amritsar 143001, Pb., India. Electronic address: prabhpreet.chem@gndu.ac.in.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2025 Mar 05; Vol. 328, pp. 125438. Date of Electronic Publication: 2024 Nov 20.
DOI: 10.1016/j.saa.2024.125438
Abstrakt: In this work, perylene diimide based radical anion (PH2 - ) is synthesized and characterized using optical, NOBF 4 methods; cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. The PH2 - is stable for 120 min (2 h) in oxygenated environment and 273 min (4.5 h) in hypoxic conditions. The PH2 - showed catalase-like activity to reduce H 2 O 2 to H 2 O with turn-over number (TON) = 20 and turn-over frequency (TOF) = 40 h -1 . The catalase-like activity can be measured using optical and electrochemical methods by monitoring the changes at 726 nm (absorbance); 585 nm (emission) and at 0.27 V. We were able to quantitatively monitor the ultra low-level concentrations of the H 2 O 2 as low as 320 fM (absorbance) and 200 fM (emission). Moreover, PH2 - could be used as a chromogenic and fluorogenic substrate for monitoring the low-level concentrations of the glucose using GOx based biochemical assay. We have demonstrated the development and validation of the glucose assay kit for the detection of glucose as low as 3.6/2.8 nM in aqueous medium and 6.2/5.6 nM in blood serum.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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