Cratylia mollis lectin reduces inflammatory burden induced by multidrug-resistant Staphylococcus aureus in diabetic wounds.

Autor: Dos Santos Silva L; Laboratório de Patogenicidade Microbiana, Universidade Ceuma, São Luís, 65075-120, Brazil., Dos Santos Castelo Branco SJ; Laboratório de Bioquímica de Proteínas, Centro de Biociências, Universidade Federal de Pernambuco, Recife, 50740-570, Brazil., Silva ISS; Laboratório de Patogenicidade Microbiana, Universidade Ceuma, São Luís, 65075-120, Brazil., Paiva MYM; Laboratório de Patogenicidade Microbiana, Universidade Ceuma, São Luís, 65075-120, Brazil., Vila Nova BG; Laboratório de Patogenicidade Microbiana, Universidade Ceuma, São Luís, 65075-120, Brazil., de Matos Chaves Lima CE; Laboratório de Odontologia, Universidade Ceuma, São Luís, 65075-120, Brazil., de Oliveira WF; Laboratório de Bioquímica de Proteínas, Centro de Biociências, Universidade Federal de Pernambuco, Recife, 50740-570, Brazil., de Paiva FEA; Departamento de Histologia, Embriologia e Biologia Celular, Universidade Federal de Goiás, Goiânia, 74690-900, Brazil., Paiva PMG; Laboratório de Bioquímica de Proteínas, Centro de Biociências, Universidade Federal de Pernambuco, Recife, 50740-570, Brazil., de Souza Monteiro A; Laboratório de Microbiologia Aplicada, Universidade Ceuma, São Luís, 65075-120, Brazil., Teixeira CS; Centro de Ciências Agrárias e da Biodiversidade, Universidade Federal do Cariri, Crato, Ceará, 63130-025, Brazil., Cardoso CG; Departamento de Histologia, Embriologia e Biologia Celular, Universidade Federal de Goiás, Goiânia, 74690-900, Brazil., Dos Santos Correia MT; Laboratório de Bioquímica de Proteínas, Centro de Biociências, Universidade Federal de Pernambuco, Recife, 50740-570, Brazil., Nascimento da Silva LC; Laboratório de Patogenicidade Microbiana, Universidade Ceuma, São Luís, 65075-120, Brazil. luisclaudionsilva@yahoo.com.br.; Laboratório de Odontologia, Universidade Ceuma, São Luís, 65075-120, Brazil. luisclaudionsilva@yahoo.com.br.
Jazyk: angličtina
Zdroj: Histochemistry and cell biology [Histochem Cell Biol] 2024 Nov 29; Vol. 163 (1), pp. 13. Date of Electronic Publication: 2024 Nov 29.
DOI: 10.1007/s00418-024-02330-9
Abstrakt: In diabetes, tissue repair is impaired, increasing susceptibility to Staphylococcus aureus infections, a pathogen commonly found in wounds. The emergence of S. aureus strains that are highly resistant to antimicrobial agents highlights the urgent need for alternative therapeutic options. One promising candidate is Cramoll (Cratylia mollis seed lectin), known for its immunomodulatory, mitogenic, and healing properties. However, its efficacy in infected diabetic wounds remains unexplored. This study evaluated the effects of topical Cramoll treatment on diabetic wounds infected by S. aureus. Diabetic Swiss mice (induced by streptozotocin) were subjected to an 8-mm wound on the back and subsequently infected with a suspension of multidrug-resistant S. aureus. During the treatment period, the wounds were clinically evaluated for inflammation and the area of injury. After seven days, samples were collected from the wounds to quantify the bacterial load and histopathological and immunological analyses. Wounds infected by S. aureus exhibited more pronounced areas and severity indices, which were significantly reduced by Cramoll treatment (p < 0.05). Histopathological analysis revealed a reduction in inflammatory cells and an increase in revascularization with Cramoll treatment (p < 0.05). Cramoll also promoted greater collagen production compared to controls (p < 0.05). Furthermore, Cramoll treatment significantly reduced the S. aureus load in wounds (p < 0.0001), decreased TNF-α and IL-6 levels in infected wounds, and increased ERK pathway activation (p < 0.05). In conclusion, Cramoll lectin improves the healing of diabetic wounds, and these results contribute to the understanding of Cramoll healing mechanisms, reinforcing its potential as a healing agent in various clinical conditions.
Competing Interests: Declarations. Conflict of interest: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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