Effect of linex treatment on IFN-γ and IL-4 in mice infected with Trichinella.

Autor: Atta SA; Department of Immunology, Theodor Bilharz Research Institute, Cairo, Egypt., Fahmy ZH; Department of Parasitology, Theodor Bilharz Research Institute, Cairo, Egypt., Selim EAH; Department of Parasitology, Theodor Bilharz Research Institute, Cairo, Egypt., Aboushousha T; Department of Pathology, Theodor Bilharz Research Institute, Cairo, Egypt., Mostafa RR; Departments of Medical Parasitology Faculty of Medicine, Cairo University, Cairo, Egypt. reham.refaat2050@kasralainy.edu.eg.
Jazyk: angličtina
Zdroj: BMC infectious diseases [BMC Infect Dis] 2024 Nov 28; Vol. 24 (1), pp. 1360. Date of Electronic Publication: 2024 Nov 28.
DOI: 10.1186/s12879-024-10202-9
Abstrakt: Trichinellosis is a zoonotic, foodborne parasitic infection causing muscle damage. This study investigated the potential therapeutic effects of the commercially available probiotic treatment Linex, both alone and in combination with Albendazole (ALB), on the intestinal and muscular stages of Trichinella spiralis infection in mice, assessing outcomes through parasitological, immunological, and histopathological measures. This study is the first to demonstrate the synergistic effect of combining the commercially available probiotic Linex with Albendazole for trichinellosis treatment. By enhancing both parasitological and immunological outcomes, this combined therapy not only significantly reduces parasite burden but also modulates the immune response, shifting it toward a protective Th1 profile. In parasitological terms, the highest adult and larval count reduction was observed in combined Linex and Albendazole treatment (100%, 97.7%) respectively. Lesser percentage of reduction were recorded in Linex alone therapy (43.2%, 88.4%) respectively. Histopathologically there was amelioration of the inflammatory cellular infiltration in all treated groups with best results in combined Linex and Albendazole treatment. Immunologically, serum IFN-γ levels increased significantly in all treated groups with highest levels in combined Linex and Albendazole treatment, while IL-4 and IL-13 level decreased significantly in all treated groups with best results observed in Linex alone treatment. To conclude; combined Linex and Albendazole treatment of mice infected with T. spirals could ameliorate the infection and improve the immune response.
Competing Interests: Declarations. Ethical approval: All experiments were conducted following the Clinical and Laboratory standards Institute (CLSI) standards and take the approval of the institution for animal ethics concerning care for animals and proper management of their waste at Theodor Bilharz Research Institute (TBRI) with approval number PT837. Consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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