Establishment of a visual assessment platform for total antioxidant capacity of Ganoderma sichuanense based on Arg-CeO 2  hydrogel nanozyme utilizing a self-programmed smartphone app and a self-designed 3D-printed device.

Autor: Tan S; School of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, China., Tan M; School of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, China., Zhang T; School of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, China., Gao L; School of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, China., Li Y; School of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, China. liyi062@yzu.edu.cn., Xu X; School of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, China. xuechaoxu@yzu.edu.cn., Yang ZQ; School of Food Science and Engineering, Yangzhou University, Yangzhou, 225127, Jiangsu, China.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Nov 29; Vol. 191 (12), pp. 770. Date of Electronic Publication: 2024 Nov 29.
DOI: 10.1007/s00604-024-06840-0
Abstrakt: A multienzyme-like L-argininemodified CeO 2  (Arg-CeO 2 ) nanozyme was prepared for accurate total antioxidant capacity (TAC) determination of Ganoderma sichuanense (G. sichuanense). It exhibits oxidase-like and peroxidase-like activities catalyzing the generation of superoxide anion free radicals and hydroxyl radicals, accelerating 3, 3', 5, 5'-tetramethylbenzidine (TMB) chromogenic reaction. Antioxidants can effectively eliminate the generated free radicals, inhibiting the TMB chromogenic reaction. Hence, TAC can be assessed by Arg-CeO 2  catalyzed TMB chromogenic reaction using ascorbic acid (AA) as a reference substance. TAC of G. sichuanense extracted using different solvents was determined, indicating the highest TAC observed in the G. sichuanense extracted by the mixture solvent (deionized water/ethanol/ethyl acetate = 1 : 1 : 1). A visual assessment platform based on Arg-CeO 2  hydrogel nanozyme utilizing a self-programmed smartphone app and a self-designed 3D printed device is established. TAC using AA as a reference substance (3.33 ~ 83.33 µM) can be detected with a detection limit as low as 0.58 µM. The platform can realize one-step quantitative determination of TAC in G. sichuanense within 40 min. The proposed visual assessment platform presented exhibits promising application prospects in the field of TAC assessment in food.
Competing Interests: Declarations. Ethical approval: This research did not involve human or animal samples. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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