Temperature-dependent luminescent copper nanoclusters with noncovalent interactions for determination of β-galactosidase activity.

Autor: Chang Y; Department of Chemistry and Environment, Jiaying University, Meisong Road 100, Meizhou, 514015, Guangdong, P. R. China., Wang J; Department of Chemistry and Environment, Jiaying University, Meisong Road 100, Meizhou, 514015, Guangdong, P. R. China., Guo H; Department of Chemistry and Environment, Jiaying University, Meisong Road 100, Meizhou, 514015, Guangdong, P. R. China., Yao W; Department of Chemistry and Environment, Jiaying University, Meisong Road 100, Meizhou, 514015, Guangdong, P. R. China., Xie H; Hangzhou Yanqu Information Technology Co., Ltd, Wen'er West Road 712, Hangzhou, 310003, Zhejiang, P. R. China., Li L; Department of Chemistry and Environment, Jiaying University, Meisong Road 100, Meizhou, 514015, Guangdong, P. R. China. alan_lee2000@163.com., Liu X; Department of Chemistry and Environment, Jiaying University, Meisong Road 100, Meizhou, 514015, Guangdong, P. R. China. liuxhv@126.com.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Nov 28; Vol. 191 (12), pp. 768. Date of Electronic Publication: 2024 Nov 28.
DOI: 10.1007/s00604-024-06844-w
Abstrakt: The synthesis of a novel bidentate ligand-protected copper nanocluster via a solid-state strategy is reported. Single-crystal X-ray diffraction analysis result reveals that the copper nanocluster features an octahedral core (Cu 6 ) coordinated by six ligands. Noncovalent interactions (C-H … π and π … π) exist between the copper nanoclusters. The copper nanocluster displays luminescence even at 250 °C. The luminescence intensity is linearly correlated with temperature changes. The copper nanocluster can assemble into luminescent nanosheets whose emission is quenched by 4-nitrophenol. Spectroscopic analysis and theoretical calculations results demonstrate that the inner filter effect and electron transfer cause the above quenching effect. A probe based on luminescent nanosheets was constructed for β-galactosidase activity determination. The linearity range is 3.3-91.8 U·L -1 , and the limit of detection is 0.45 U·L -1 . This probe was also evaluated for determination of the β-galactosidase activity in human serum via spiking experiments. The recoveries ranged from 96.2% to 101.8%.
Competing Interests: Declarations. Competing Interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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