Biopolymer-based oral films integrated with probiotic active compounds for improved health applications.

Autor: Tunçer Çağlayan S; Vocational School of Health Services, Department of Medical Services and Techniques, Bilecik Şeyh Edebali University, Bilecik, 11100, Turkey. sinem.tuncer@bilecik.edu.tr.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2024 Nov 28; Vol. 207 (1), pp. 4. Date of Electronic Publication: 2024 Nov 28.
DOI: 10.1007/s00203-024-04207-w
Abstrakt: Orally dissolving films (ODFs) have emerged as a versatile platform that combines convenience, efficacy, and patient compliance. In this study, the cell-free supernatant of the oral probiotic Streptococcus salivarius M18 was incorporated into various biopolymer-based ODF formulations, evaluated for demolding, fragility, and flexibility. The combination of carboxymethyl cellulose, sodium alginate, and glycerol successfully formed stable films. The films were characterized by weight, thickness, pH, and disintegration times. Fourier-transform infrared spectroscopy (FTIR) was used to analyze ODF content and release profiles in simulated saliva. Unique absorption peaks in the cell-free product-incorporated ODF samples confirmed the integration of bacterial proteins, lipids, and nucleic acids into the ODF matrix. The biological activity of the ODF carrying M18 bioactive products was assessed by its inhibitory effect on the growth of Streptococcus mutans, a pathogen linked to dental plaque and cavities. Additionally, the anti-proliferative effect on cancer epithelial cells was demonstrated. This study show that probiotic products can be integrated into bio-based thin films without losing activity, making this delivery platform promising for local and potentially systemic effects.
Competing Interests: Declarations. Ethical approval: This project does not contain any studies with human or animal subjects. Informed consent: For this type of study, consent is not required. Competing interests: The authors declare no competing interests. Conflict of interest: The author declares no competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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