Geometrically modified bovine pericardium membrane promotes the expression of molecules targeted for a faster integration and vascularization process.

Autor:	Morgante O; Department of Innovative Technologies in Medicine and Dentistry, University 'G. d'Annunzio' Chieti-Pescara, Chieti, Italy., Della Rocca Y; Department of Innovative Technologies in Medicine and Dentistry, University 'G. d'Annunzio' Chieti-Pescara, Chieti, Italy., Marconi GD; Department of Innovative Technologies in Medicine and Dentistry, University 'G. d'Annunzio' Chieti-Pescara, Chieti, Italy., Mazzone A; Department of Innovative Technologies in Medicine and Dentistry, University 'G. d'Annunzio' Chieti-Pescara, Chieti, Italy., Cavalcanti MFXB; Department of Dental Clinic, Universidade Nove de Julho, SãoPaulo, Brazil., Trubiani O; Department of Innovative Technologies in Medicine and Dentistry, University 'G. d'Annunzio' Chieti-Pescara, Chieti, Italy., Diomede F; Department of Innovative Technologies in Medicine and Dentistry, University 'G. d'Annunzio' Chieti-Pescara, Chieti, Italy., Pizzicannella J; Department of Engineering and Geology, University 'G. d'Annunzio' Chieti-Pescara, Pescara, Italy.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2024 Nov 13; Vol. 12, pp. 1455215. Date of Electronic Publication: 2024 Nov 13 (Print Publication: 2024).
DOI:	10.3389/fbioe.2024.1455215
Abstrakt:	Introduction: In recent years, advancements in technology and the refinement of engineering techniques have facilitated the development of tissue engineering, placing particular emphasis on the use of 3D-biomaterials with several structural and chemical geometric features. In particular, increasing information on biomaterial geometric surfaces has allowed for a better understanding of tissue regenerative processes. In the present study a comparison between BioRipar ® , bovine pericardium membrane, modified with micrometric roundish regular open pores (BioR-Ps) and BioRipar ® without pores (BioR-NPs) has been investigated. Methods: The expression of adhesion molecules such as: fibronectin, vimentin, focal adhesion kinase (FAK), integrin 1β, integrin α5, E-cadherin, and molecules involved in neovascularization processes such as: vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor (VEGF-R) were evaluated in an in vitro model containing primary culture of human periodontal ligament stem cells (hPDLSCs) through multiparametric analysis. Results: The results indicated a markedly significant expression of all the abovementioned molecules in hPDLSCs cultured withBioR-Ps compared to hPDLSCs cultured with BioR-NPs. Scanning electron microscopy analysis indicated a marked interaction between the cells and the substrate, particularly evident in the proximity of open pores in the hPDLSCs cultured on the BioR-P surface compared to hPDLSCs cultured on the BioR-NP surface. Thus, the presence of micrometric open pores on the scaffold stimulates the proliferation potential of cells apart from their adhesion ability on the patch, particularly near the pores. Discussion: Expression of angiogenic molecules strengthened the performance of the modified BioR-Ps. During synthesis, 3D-biomaterial micrometric open-pores enable better bonding between cells and materials, increasing contact area and promoting cellular molecular signals in biomaterial-guided tissue engineering. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Morgante, Della Rocca, Marconi, Mazzone, Cavalcanti, Trubiani, Diomede and Pizzicannella.)
Databáze:	MEDLINE
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