Pathological microcircuits initiate epileptiform events in patient hippocampal slices.

Autor:	Elliott MAT; Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, USA.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA., Andrews JP; Department of Neurological Surgery, University of California San Francisco, San Francisco, CA, USA., van der Molen T; Neuroscience Research Institute, University of California Santa Barbara, Santa Barbara, CA, USA.; Department of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA, USA., Geng J; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA.; Department of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA, USA., Spaeth A; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA.; Department of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA, USA., Voituik K; Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, USA.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA., Core C; Scientific Software Engineering Center, eScience Institute, University of Washington, Seattle, WA USA., Gillespie T; Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, USA.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA., Sinervo A; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA., Parks DF; Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, USA.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA., Robbins A; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA.; Department of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA, USA., Solís D; Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, USA.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA., Chang EF; Department of Neurological Surgery, University of California San Francisco, San Francisco, CA, USA.; Weill Institute of Neurosciences, University of California San Francisco, San Francisco, CA, USA., Nowakowski TJ; Department of Neurological Surgery, University of California San Francisco, San Francisco, CA, USA.; Weill Institute of Neurosciences, University of California San Francisco, San Francisco, CA, USA.; Department of Psychiatry and Behavioral Sciences, University of California San Francisco, San Francisco, CA, USA.; The Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California San Francisco, San Francisco, CA, USA., Teodorescu M; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA.; Department of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA, USA., Haussler D; Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, USA.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA., Sharf T; Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, USA.; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Nov 14. Date of Electronic Publication: 2024 Nov 14.
DOI:	10.1101/2024.11.13.623525
Abstrakt:	How seizures begin at the level of microscopic neural circuits remains unknown. High-density CMOS microelectrode arrays provide a new avenue for investigating neuronal network activity, with unprecedented spatial and temporal resolution. We use high-density CMOS-based microelectrode arrays to probe the network activity of human hippocampal brain slices from six patients with mesial temporal lobe epilepsy in the presence of hyperactivity promoting media. Two slices from the dentate gyrus exhibited epileptiform activity in the presence of low magnesium media with kainic acid. Both slices displayed an electrophysiological phenotype consistent with a reciprocally connected circuit, suggesting a recurrent feedback loop is a key driver of epileptiform onset. Larger prospective studies are needed, but these findings have the potential to elucidate the network signals underlying the initiation of seizure behavior. Competing Interests: Competing interests: All authors declare no competing interests.
Databáze:	MEDLINE
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