Determination of Pseudomonas aeruginosa MexXY-OprM substrate profile in a major efflux knockout system reveals distinct antibiotic substrate classes.
| Autor: | Kavanaugh LG; Department of Biochemistry, Emory University School of Medicine, Atlanta, GA.; Microbiology and Molecular Genetics Graduate Program, Emory University, Atlanta, GA., Hariharan SM; Department of Biochemistry, Emory University School of Medicine, Atlanta, GA., Conn GL; Department of Biochemistry, Emory University School of Medicine, Atlanta, GA.; Microbiology and Molecular Genetics Graduate Program, Emory University, Atlanta, GA.; Emory Antibiotic Resistance Center, Emory University, Atlanta, GA. |
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| Jazyk: | angličtina |
| Zdroj: | BioRxiv : the preprint server for biology [bioRxiv] 2024 Nov 20. Date of Electronic Publication: 2024 Nov 20. |
| DOI: | 10.1101/2024.10.18.619101 |
| Abstrakt: | Defining the substrates of Resistance-Nodulation-Division (RND) systems is often complicated by the presence of multiple systems in a single bacterial species. Using a major efflux knockout strain, we developed a Pseudomonas aeruginosa system that enables controlled expression of MexXY-OprM in the absence of background efflux complexes with overlapping substrate profiles. This system identified three groups of potential substrates: substrates, partial substrates, and nonsubstrates, and defined trimethoprim as a new substrate for MexXY-OprM. |
| Databáze: | MEDLINE |
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