Survey and genomic characterization of Serratia marcescens on endophytism, biofilm, and phosphorus solubilization in rice plants.

Autor: de Oliveira RS; Agronomy School, Federal University of Goiás, Goiânia, Goiás, 74001-970, Brazil.; Agricultural Microbiology Laboratory, Brazilian Agricultural Research Corporation Rice and Beans (Embrapa Arroz E Feijão), Rodovia GO-462, Km 12, Fazenda Capivara, Zona Rural, Caixa Postal: 179, Santo Antônio de Goiás, Goiás, CEP: 75375-000, Brazil., Gonçalves AR; Agricultural Microbiology Laboratory, Brazilian Agricultural Research Corporation Rice and Beans (Embrapa Arroz E Feijão), Rodovia GO-462, Km 12, Fazenda Capivara, Zona Rural, Caixa Postal: 179, Santo Antônio de Goiás, Goiás, CEP: 75375-000, Brazil., Ajulo AA; Agronomy School, Federal University of Goiás, Goiânia, Goiás, 74001-970, Brazil.; Agricultural Microbiology Laboratory, Brazilian Agricultural Research Corporation Rice and Beans (Embrapa Arroz E Feijão), Rodovia GO-462, Km 12, Fazenda Capivara, Zona Rural, Caixa Postal: 179, Santo Antônio de Goiás, Goiás, CEP: 75375-000, Brazil., Oliveira LR; Agronomy School, Federal University of Goiás, Goiânia, Goiás, 74001-970, Brazil.; Agricultural Microbiology Laboratory, Brazilian Agricultural Research Corporation Rice and Beans (Embrapa Arroz E Feijão), Rodovia GO-462, Km 12, Fazenda Capivara, Zona Rural, Caixa Postal: 179, Santo Antônio de Goiás, Goiás, CEP: 75375-000, Brazil., Lanna AC; Agricultural Microbiology Laboratory, Brazilian Agricultural Research Corporation Rice and Beans (Embrapa Arroz E Feijão), Rodovia GO-462, Km 12, Fazenda Capivara, Zona Rural, Caixa Postal: 179, Santo Antônio de Goiás, Goiás, CEP: 75375-000, Brazil., de Filippi MCC; Agricultural Microbiology Laboratory, Brazilian Agricultural Research Corporation Rice and Beans (Embrapa Arroz E Feijão), Rodovia GO-462, Km 12, Fazenda Capivara, Zona Rural, Caixa Postal: 179, Santo Antônio de Goiás, Goiás, CEP: 75375-000, Brazil. cristina.filippi@embrapa.br.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2024 Dec; Vol. 31 (57), pp. 65834-65848. Date of Electronic Publication: 2024 Nov 28.
DOI: 10.1007/s11356-024-35554-2
Abstrakt: Serratia marcescens, isolated from the rhizosphere of rice crops, has the potential to improve the acquisition of scarce minerals and provide plant growth. Rice seeds microbiolized with S. marcescens and non-microbiolized seeds were sown in a culture medium enriched with non-labile phosphorus, and the roots were analyzed in WinRhizo. The plant segments were documented by scanning electron microscopy (SEM) and incubated in an NBRIP culture medium. DNAs from endophytic colonies were extracted and analyzed by PCR. The genome of S. marcescens was annotated using subsystem technology to detect genes involved in phosphorus solubilization, biofilm production, and growth promotion. The root system increased in area, volume, and length by 61.5, 31.5, and 101%, respectively. Halos were formed around segments of microbiolized plants, indicating the solubilization of non-labile phosphorus. SEM detected the presence of biofilms and microcolonies, identified as S. marcescens by the molecular markers. Genome annotation found genes with potential functions in plant growth promotion, including genes involved in the biosynthesis of indole-3-acetic acid, phosphate solubilization, and biofilm production. In the low phosphorus crop, the treated plants showed a 181% increase in total biomass. S. marcescens solubilizes non-labile phosphorus, colonizes endophytes, modifies the architecture of the root system, and promotes the growth of rice plants, and can be considered a biofertilizer for growing upland rice.
Competing Interests: Declarations. Ethical approval: Not applicable. Consent to participate: Not applicable. Consent for publication: All authors were allowed to read and approve the final manuscript. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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