Recessivity of deltamethrin resistance, kdr mutations, and detoxifying enzymes in hybrids of Triatoma phyllosoma subcomplex from Mexico.

Autor: Davila-Barboza JA; Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon (UANL), Av. Universidad S/N Cd. Universitaria, N.L. 66455, San Nicolas de los Garza, Mexico., Gutierrez-Rodriguez SM; Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon (UANL), Av. Universidad S/N Cd. Universitaria, N.L. 66455, San Nicolas de los Garza, Mexico., Lopez-Monroy B; Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon (UANL), Av. Universidad S/N Cd. Universitaria, N.L. 66455, San Nicolas de los Garza, Mexico., Fernandez-Salas I; Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon (UANL), Av. Universidad S/N Cd. Universitaria, N.L. 66455, San Nicolas de los Garza, Mexico., Rodriguez-Sanchez IP; Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon (UANL), Av. Universidad S/N Cd. Universitaria, N.L. 66455, San Nicolas de los Garza, Mexico., Villegas-Trejo A; Consultoria para la Evaluacion e Investigacion en Salud Publica (CEISP), Fraccionamiento B y San Miguel Acapatzingo, Privada Las Flores, 62446, Cuernavaca, Morelos, Mexico., Flores AE; Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon (UANL), Av. Universidad S/N Cd. Universitaria, N.L. 66455, San Nicolas de los Garza, Mexico. adriana.floressr@uanl.edu.mx.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2024 Nov 28; Vol. 123 (11), pp. 399. Date of Electronic Publication: 2024 Nov 28.
DOI: 10.1007/s00436-024-08396-7
Abstrakt: Triatoma species from the phyllosoma subcomplex are sympatrically distributed and include some of the main vectors of Chagas disease in Mexico. Species within this subcomplex, including Triatoma pallidipennis, T. mazzottii, T. picturata, and T. longipennis, have shown resistance to pyrethroid insecticides, associated with mutations in the para gene of the voltage gate sodium channel (VGSC) and the activity of detoxifying enzymes such as β-esterases and glutathione s-transferases (GST). In this study, we evaluated resistance to deltamethrin in hybrids of T. pallidipennis × T. mazzottii (T.pal × T.maz) and T. pallidipennis × T. picturata (T.pal × T.pic) under laboratory conditions, and the inheritance was determined based on the degree of dominance (DO). Additionally, associated resistance mechanisms were analyzed, including detoxifying enzymes and knockdown resistance (kdr) mutations. High levels of resistance to deltamethrin were found in the hybrids of T.pal × T.maz when compared with the susceptible strain of T. mazzottii (RR 50  = 17.50). Dominance levels calculated for each hybrid showed values <  - 1, confirming that resistance to deltamethrin was recessive. Hybrids exhibited reduced α-, β-esterases, and cytochrome P 450 mixed-function oxidases (MFO) activity. However, both hybrids showed significantly increased GST activity, particularly in T.pal × T.pic, suggesting enhanced detoxification through this pathway. The kdr mutation A943V, present in T. mazzottii, was found in T.pal × T.maz hybrids. These results emphasize the importance of considering hybridization in resistance management programs and its potential impact on the success of insecticide-based control measures.
Competing Interests: Declarations. Ethics approval: Not applicable. Consent to participate: Not applicable. Clinical trial number: Not applicable. Consent for publication: Not applicable. Conflict of interest: The author have no relevant financial or non-financial interests to disclose.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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