Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate.

Autor:	Bertrand Q; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland., Nogly P; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland.; Institute of Molecular Biology and Biophysics, Department of Biology, ETH Zürich, Zürich, Switzerland.; Dioscuri Center for Structural Dynamics of Receptors, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Krakow, Poland., Nango E; RIKEN Spring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, Japan.; Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, Japan.; Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto, Japan., Kekilli D; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland., Khusainov G; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland., Furrer A; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland., James D; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland., Dworkowski F; Photon Science Division, Paul Scherrer Institut, Villigen, Switzerland., Skopintsev P; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland., Mous S; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland.; Institute of Molecular Biology and Biophysics, Department of Biology, ETH Zürich, Zürich, Switzerland., Martiel I; Photon Science Division, Paul Scherrer Institut, Villigen, Switzerland., Börjesson P; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, Gothenburg, Sweden., Ortolani G; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, Gothenburg, Sweden., Huang CY; Photon Science Division, Paul Scherrer Institut, Villigen, Switzerland., Kepa M; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland., Ozerov D; Photon Science Division, Paul Scherrer Institut, Villigen, Switzerland., Brünle S; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland., Panneels V; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland., Tanaka T; RIKEN Spring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, Japan., Tanaka R; RIKEN Spring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, Japan., Tono K; Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, Japan., Owada S; RIKEN Spring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo, Japan., Johnson PJM; Photon Science Division, Paul Scherrer Institut, Villigen, Switzerland., Nass K; Photon Science Division, Paul Scherrer Institut, Villigen, Switzerland., Knopp G; Photon Science Division, Paul Scherrer Institut, Villigen, Switzerland., Cirelli C; Photon Science Division, Paul Scherrer Institut, Villigen, Switzerland., Milne C; Photon Science Division, Paul Scherrer Institut, Villigen, Switzerland., Schertler G; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland., Iwata S; Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto, Japan.; Japan Science and Technology Agency (JST)-Precursory Research for Embryonic Science and Technology (PRESTO), 4-1-8 Honcho, Kawaguchi, Saitama, Japan., Neutze R; Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462, Gothenburg, Sweden., Weinert T; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland. tobias.weinert@psi.ch., Standfuss J; Division of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland. joerg.standfuss@psi.ch.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2024 Nov 27; Vol. 15 (1), pp. 10278. Date of Electronic Publication: 2024 Nov 27.
DOI:	10.1038/s41467-024-54422-8
Abstrakt:	Time-resolved serial crystallography at X-ray Free Electron Lasers offers the opportunity to observe ultrafast photochemical reactions at the atomic level. The technique has yielded exciting molecular insights into various biological processes including light sensing and photochemical energy conversion. However, to achieve sufficient levels of activation within an optically dense crystal, high laser power densities are often used, which has led to an ongoing debate to which extent photodamage may compromise interpretation of the results. Here we compare time-resolved serial crystallographic data of the bacteriorhodopsin K-intermediate collected at laser power densities ranging from 0.04 to 2493 GW/cm 2 and follow energy dissipation of the absorbed photons logarithmically from picoseconds to milliseconds. Although the effects of high laser power densities on the overall structure are small, in the upper excitation range we observe significant changes in retinal conformation and increased heating of the functionally critical counterion cluster. We compare light-activation within crystals to that in solution and discuss the impact of the observed changes on bacteriorhodopsin biology. Competing Interests: Competing interests: The authors declare no competing interests. (© 2024. The Author(s).)
Databáze:	MEDLINE
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