Responsive 3D Printed Microstructures Based on Collagen Folding and Unfolding.

Autor: Mainik P; Institute for Molecular Systems Engineering and Advanced Materials (IMSEAM), Heidelberg University, 69120, Heidelberg, Germany.; Organic Chemistry Institute (OCI), Heidelberg University, 69120, Heidelberg, Germany., Aponte-Santamaría C; Heidelberg Institute for Theoretical Studies (HITS), 69118, Heidelberg, Germany., Fladung M; Cell and Neurobiology, Zoological Institute, Karlsruhe Institute of Technology (KIT), 76131, Karlsruhe, Germany., Curticean RE; BioQuant, Heidelberg University, 69120, Heidelberg, Germany., Wacker I; BioQuant, Heidelberg University, 69120, Heidelberg, Germany., Hofhaus G; BioQuant, Heidelberg University, 69120, Heidelberg, Germany., Bastmeyer M; Cell and Neurobiology, Zoological Institute, Karlsruhe Institute of Technology (KIT), 76131, Karlsruhe, Germany.; Institute for Biological and Chemical Systems - Biological Information Processing (IBCS-BIP), Karlsruhe Institute of Technology (KIT), 76344, Karlsruhe, Germany., Schröder RR; BioQuant, Heidelberg University, 69120, Heidelberg, Germany., Gräter F; Heidelberg Institute for Theoretical Studies (HITS), 69118, Heidelberg, Germany.; Interdisciplinary Center for Scientific Computing (IWR), Heidelberg University, 69120, Heidelberg, Germany., Blasco E; Institute for Molecular Systems Engineering and Advanced Materials (IMSEAM), Heidelberg University, 69120, Heidelberg, Germany.; Organic Chemistry Institute (OCI), Heidelberg University, 69120, Heidelberg, Germany.
Jazyk: angličtina
Zdroj: Small (Weinheim an der Bergstrasse, Germany) [Small] 2024 Nov 27, pp. e2408597. Date of Electronic Publication: 2024 Nov 27.
DOI: 10.1002/smll.202408597
Abstrakt: Mimicking extracellular matrices holds great potential for tissue engineering in biological and biomedical applications. A key compound for the mechanical stability of these matrices is collagen, which also plays an important role in many intra- and intercellular processes. Two-photon 3D laser printing offers structuring of these matrices with subcellular resolution. So far, efforts on 3D microprinting of collagen have been limited to simple geometries and customized set-ups. Herein, an easily accessible approach is presented using a collagen type I methacrylamide (ColMA) ink system which can be stored at room temperature and be precisely printed using a commercial two-photon 3D laser printer. The formulation and printing parameters are carefully optimized enabling the manufacturing of defined 3D microstructures. Furthermore, these printed microstructures show a fully reversible response upon heating and cooling in multiple cycles, indicating successful collagen folding and unfolding. This experimental observation has been supported by molecular dynamics simulations. Thus, the study opens new perspectives for designing new responsive biomaterials for 4D (micro)printing.
(© 2024 The Author(s). Small published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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