Curcumin mitigates sodium fluoride toxicity in Caenorhabditis elegans.

Autor: Xu J; School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China; Department of Rehabilitation, First Medical Center, Chinese PLA General Hospital, Beijing 100853, China., Zhang B; School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China., Liu X; School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China., Du P; School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China., Wang W; School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China., Zhang C; School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488, China; Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang 550004, China. Electronic address: jxs@bucm.edu.cn.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2024 Dec; Vol. 288, pp. 117372. Date of Electronic Publication: 2024 Nov 26.
DOI: 10.1016/j.ecoenv.2024.117372
Abstrakt: Fluoride, a naturally occurring element found in water, soil, food, and atmospheric precipitation, can lead to fluorosis and various health issues when consumed excessively. However, the mechanism of fluorosis is still under investigation. This study utilizes Caenorhabditis elegans as a model organism to investigate the effects of fluoride exposure on biological systems and to explore the mechanisms by which curcumin mitigates fluoride-induced toxicity. Three groups were established: a blank control, a sodium fluoride (NaF) exposure group (concentration 5 mmol/L), and a curcumin intervention group (concentration 25 μmol/L). Physiological parameters, lipofuscin levels, intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential, and mitochondrial copy numbers were measured to assess the effects of fluoride toxicity and curcumin protection. RNA-seq and qRT-PCR were utilized to investigate the molecular mechanisms underlying fluoride-induced damage and curcumin's mitigating effects. Results indicated that fluoride-exposed nematodes displayed physiological abnormalities, increased ROS production, higher lipofuscin levels, altered mitochondrial membrane potential and mitochondrial copy number, and activated MAPK signaling pathway genes. Curcumin exhibited protective effects on these parameters, suggesting its potential in preventing fluoride-induced harm by modulating oxidative stress and preserving mitochondrial function. This research enhances our understanding of the mechanisms of fluoride toxicity and highlights the potential benefits of curcumin.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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